Biocompatibility studies of natural rubber latex from different tree clones and collection methods
Biocompatibility studies of natural rubber latex from different tree clones and collection methods
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不同克隆树天然胶乳的生物相容性研究和收集方法
DOI：
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发表时间：
2014
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通讯作者：
Carlos Frederico de Oliveira Graeff
中科院分区：
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作者：
J. Floriano;L. S. Mota;E. Furtado;V. J. Rossetto;Carlos Frederico de Oliveira Graeff
天然胶乳（NRL）有几个特点，使其成为一种极好的生物材料，促进组织、皮肤和骨骼的生长和修复。大多数对NRL膜的研究在收集过程中使用了不同克隆和化学防腐剂的混合物。在这项研究中，我们比较了产生NRL的五个克隆，试图确定它们在生物相容性方面的差异。研究的无性系为巴西人工林中常见的RRIM 600、PB 235、GT1、PR 255和IAN 873。我们还研究了在乳胶收集过程中使用氨的效果。NRL膜是无菌制备和灭菌的。在体外实验中，膜与小鼠成纤维细胞直接接触24、48和72小时。在体内实验中，膜被皮下植入家兔。结果表明，从所有克隆中获得的膜具有良好的生物相容性。来自RRIM 600和IAN 873克隆的膜诱导了更大的细胞增殖，表明更大的生物活性。研究发现，在收集过程中，乳胶制成的膜与氨接触，在培养物中表现出细胞毒性和基因毒性作用，以及坏死，并增加了靠近植入物的兔子组织中的炎症细胞。
Natural rubber latex (NRL) has several features that make it an excellent biomaterial to promote the growth and repair of tissues, skin and bones. Most of the research with NRL membranes uses a mixture of different clones and chemical preservatives in the collection process. In this study, we compared five clones that produce NRL, seeking to identify their differences in biocompatibility. The clones studied were RRIM 600, PB 235, GT1, PR 255 and IAN 873 commonly found in plantations in Brazil. We did also study the effect of ammonia used during latex collection. NRL membranes were prepared aseptically and sterilized. In the in vitro tests, the membranes remained in direct contact with mouse fibroblasts cells for three periods, 24, 48 and 72 h. In the in vivo tests, the membranes were implanted subcutaneously in rabbits. The results indicated the biocompatibility of the membranes obtained from all clones. Membranes from the clones RRIM 600 and IAN 873 induced greater cell proliferation, suggesting greater bioactivity. It was found that the membranes made from latex that was in contact with ammonia during collection, showed cytotoxic and genotoxic effects in cultures, as well as necrosis, and increased inflammatory cells in the rabbit’s tissues close to the implant.