Three- and Four-Dimensional Heteronuclear NMR
Three- and Four-Dimensional Heteronuclear NMR
复制标题
三维和四维异核核磁共振
DOI：
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
A. Gronenborn
中科院分区：
文献类型：
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作者：
G. Clore;A. Gronenborn
用于核磁共振解析大分子三维结构的几何信息的主要来源是由核Overhauser增强(NOE)测量(1-5)得到的短(~lt；5A)近似质子间距离约束。为了提取这一信息，首先必须完全指定所讨论的大分子的1H谱，然后指定尽可能多的结构上有用的NOE相互作用。NOE约束的数目越大，得到的结构的精度和准确度就越高(5-7)。事实上，用目前最先进的方法，现在有可能获得蛋白质的核磁共振结构，其精度和准确度可与2 A分辨率的晶体结构相媲美(7-9)。
The principal source of geometric information used to solve three dimensional structures of macromolecules by NMR resides in short (< 5A) approximate interproton distance restraints derived from nuclear Overhauser enhancement (NOE) measurements (1–5). In order to extract this information it is essential to first completely assign the 1H spectrum of the macromolecule in question and then to assign as many structurally useful NOE interactions as possible. The larger the number of NOE restraints, the higher the precision and accuracy of the resulting structures (5–7). Indeed, with current state-of-the-art methodology it is now possible to obtain NMR structures of proteins at a precision and accuracy comparable to 2 A resolution crystal structures (7–9)