POSSIBLE ROLE OF OSTEOPONTIN IN DEPOSITION OF CALCIUM-PHOSPHATE IN HUMAN PILOMATRICOMAS
POSSIBLE ROLE OF OSTEOPONTIN IN DEPOSITION OF CALCIUM-PHOSPHATE IN HUMAN PILOMATRICOMAS
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DOI：
10.1111/1523-1747.ep12313472
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发表时间：
1995-07-01
影响因子：
6.5
通讯作者：
KITAMURA, Y
中科院分区：
文献类型：
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作者：
HIROTA, S;ASADA, H;KITAMURA, Y
人毛发瘤是一种良性的表皮附属物肿瘤，由毛发基质样基底样细胞和角化残留细胞组成，称为影子细胞。磷酸钙的沉积通常发生在影子细胞巢中。因为骨桥蛋白被认为与骨骼中磷酸钙的沉积有关，所以我们询问骨桥蛋白信使RNA是否也在毛发瘤中表达。应用Northern杂交和原位杂交技术，我们在毛发瘤组织中检测到骨桥蛋白信使RNA，而在正常皮肤组织中未检测到。采用原位杂交和免疫组织化学相结合的方法，发现表达骨桥蛋白信使RNA的细胞为位于影子细胞巢周围的CD68阳性巨噬细胞，抗人骨桥蛋白抗体免疫组织化学显示骨桥蛋白的定位与磷酸钙的定位一致。本研究结果提示，巨噬细胞产生的骨桥蛋白可能在毛母细胞瘤影子细胞巢内钙磷沉积过程中起重要作用。
Human pilomatricomas are benign epidermal appendage tumors composed of hair matrix-like basaloid cells and keratinized remnant cells referred to as shadow cells. Deposition of calcium phosphate usually occurs in the shadow cell nests. Because osteopontin is believed to be involved in the deposition of calcium phosphate in bones, we asked whether osteopontin messenger RNA also is expressed in pilomatricomas. Using Northern blotting and in sits hybridization we detected osteopontin messenger RNA in pilomatricoma tissues but not in normal skin tissue. By the combination of in situ hybridization and immunohistochemistry, osteopontin messenger RNA-expressing cells were identified as CD68-positive macrophages surrounding the shadow cell nests, Immunohistochemistry of anti-human osteopontin antibodies revealed that the localization of osteopontin protein was consistent with that of calcium phosphate. The present results suggest that osteopontin produced by macrophages may play a significant role in the deposition of calcium phosphate in the shadow cell nests of pilomatricomas.