Elevated expression of IL-17 and IL-12 genes in chronic inflammatory periodontal disease
Elevated expression of IL-17 and IL-12 genes in chronic inflammatory periodontal disease
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DOI：
10.1016/j.cca.2008.06.003
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发表时间：
2008-09-01
影响因子：
5
通讯作者：
Yamazaki, Kazuhisa
中科院分区：
文献类型：
--
作者：
Honda, Tomoyuki;Aoki, Yukari;Yamazaki, Kazuhisa
背景资料：许多不同的理论已经被假定来解释牙龈炎的进展牙周炎的背景下，Th 1/Th 2的范例。然而，没有得到一致的结果。Th 17是一种产生IL-17的新的T细胞亚群，它参与炎症组织破坏的许多方面，克服了与Th 1/Th 2假说相关的研究中的许多不一致的发现。我们比较了牙龈炎和牙周炎病变中Th 17相关分子的基因表达谱，显示出不同的临床Entities.Methods：牙龈组织样本，从23牙龈炎和24牙周炎组织。通过使用定量实时PCR测量IL-17 A、IL-17 F、CCR 4、CCR 6、IL-12 p35和IL-23 p19的基因表达。采用Mann-Whitney U检验分析牙龈炎和牙周炎组织中基因表达的差异。结果：IL-17 A的表达水平高于IL-17 F，且IL-17 A在牙龈炎和牙周炎中的表达差异有统计学意义。与牙龈炎相比，CCR 4和CCR 6在牙周炎中倾向于更高，尽管差异无统计学意义。IL-12 p35基因表达在牙周炎中明显高于牙龈炎，而IL-23 p19基因表达在牙周炎和牙龈炎中无明显差异。与牙龈炎相比，牙周病的组织破坏形式，并为牙周病的T细胞介导的免疫发病机制提供了新的见解。(C)2008 Elsevier B. V.保留所有权利。
Background: A number of different theories have been postulated to explain the progression of gingivitis to periodontitis in the context of the Th1/Th2 paradigm. However, no consistent results have been obtained. Th17, a new T-cell subset producing IL-17, which is implicated in many aspect of inflammatory tissue destruction, overcomes many of the discrepant findings in the studies related to the Th1/Th2 hypothesis. We compared the gene expression profile of Th17-related molecules in gingivitis and periodontitis lesions showing distinct clinical entities.Methods: Gingival tissue samples were obtained from 23 gingivitis and 24 periodontitis tissues. The gene expression was measured by using quantitative real-time PCR for IL-17A, IL-17F, CCR4, CCR6, IL-12 p35 and IL-23 p19. The difference of gene expressions between gingivitis and periodontitis was analyzed by Mann-Whitney U-test. Correlations between each gene expression were also analyzed.Results: The expression level of IL-17A was higher than that of IL-17F and a significant difference in expression between gingivitis and periodontitis was observed only for IL-17A. CCR4 and CCR6 tended to be higher in periodontitis compared with gingivitis, although the differences were not statistically significant. Whereas the gene expression of IL-12 p35 was significantly higher in periodontitis compared with gingivitis, that of IL-23 p19 was not different between the two diseases.Conclusion: This study demonstrates the elevated expression of IL-17 and IL-12 in periodontitis, i.e.. the tissue destruction form of periodontal diseases, as compared with gingivitis, and provides new insight into the T-cell mediated immunopathogenesis of periodontal disease. (C) 2008 Elsevier B.V. All rights reserved.