INHIBITORS OF DYNEIN ACTIVITY BLOCK INTRACELLULAR-TRANSPORT IN ERYTHROPHORES
INHIBITORS OF DYNEIN ACTIVITY BLOCK INTRACELLULAR-TRANSPORT IN ERYTHROPHORES
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DOI：
10.1038/295701a0
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发表时间：
1982-01-01
期刊：
影响因子：
64.8
通讯作者：
PORTER, KR
中科院分区：
文献类型：
--
作者：
BECKERLE, MC;PORTER, KR
红细胞是一种能够沿着放射状排列的细胞骨架转运色素颗粒的细胞，为研究细胞内颗粒转运提供了一个很好的模型系统。细胞内运动的许多例子是基于微管1和所有现有的证据表明，有组织的色素颗粒运输在红细胞依赖于这些结构2。研究得最好的基于微管的运动系统是鞭毛。由于它依赖于动力蛋白-微管相互作用产生动力3，我们研究了动力蛋白ATP酶抑制剂对色素颗粒转运的影响。钒酸盐是体外动力蛋白ATP酶活性的有效抑制剂4 - 6，并已显示出抑制纤毛4、鞭毛4和有丝分裂纺锤体7中基于微管的运动。最近，已经描述了另一种动力蛋白抑制剂，β-9-3-（2-羟基壬基）腺嘌呤（EHNA）8。我们在这里报告，微量注射钒酸盐和外源性应用EHNA块跳跃运动的色素颗粒在孤立的松鼠鱼红细胞。钒酸盐和EHNA定向色素运动的敏感性表明，动力蛋白样分子可能是红细胞内运动系统的重要组成部分。
Erythrophores, cells capable of translocating pigment granules along a radially arrayed cytoskeletal framework, provide an excellent model system for studying intracellular particle transport. Numerous examples of intracellular motility are based on microtubules1and all available evidence indicates that organized pigment granule transport in erythrophores depends on these structures2. The best studied microtubule-based motility system is the flagellum. As it relies on a dynein–microtubule interaction to generate motive force3, we have investigated the effects of dynein ATPase inhibitors on pigment granule transport. Vanadate is a potent inhibitor of dynein ATPase activityin vitro4–6and has been shown to inhibit microtubule-based motility in cilia4, flagella4and the mitotic spindle7. Recently, another dynein inhibitor, erythro-9-3-(2-hydroxynonyl)adenine (EHNA), has been described8. We report here that both microinjected vanadate and exogenously applied EHNA block saltatory movement of pigment granules in isolated squirrelfish erythrophores. The vanadate and EHNA sensitivities of directed pigment motion suggest that a dynein-like molecule may be an essential component of the erythrophore intracellular motility system.