Production of Lycopene in the Non-Carotenoid-Producing Yeast Yarrowia lipolytica
Production of Lycopene in the Non-Carotenoid-Producing Yeast Yarrowia lipolytica
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DOI：
10.1128/aem.03167-13
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发表时间：
2014-03-01
影响因子：
4.4
通讯作者：
Barth, Gerold
中科院分区：
文献类型：
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作者：
Matthaeus, Falk;Ketelhot, Markus;Barth, Gerold
在解脂耶氏酵母TEF 1启动子的控制下，将密码子优化的菠萝泛菌crtB和crtI基因在解脂耶氏酵母中表达。解脂的此外，还对Y.过表达解脂菌GGS 1和HMG 1，以增加番茄红素的产生。所有的基因也在Y. POX 1至POX 6和GUT 2缺失的解脂菌菌株，这导致脂质体的大小增加和番茄红素产量的进一步增加。番茄红素主要存在于脂质体中，脂质体形成的增加导致Y.解脂生长限制条件增加了特定的番茄红素含量。最后，在补料分批培养中达到16 mg g（-1）（干细胞重量）的产量，这是迄今为止报道的真核宿主的最高值。
The codon-optimized genes crtB and crtI of Pantoea ananatis were expressed in Yarrowia lipolytica under the control of the TEF1 promoter of Y. lipolytica. Additionally, the rate-limiting genes for isoprenoid biosynthesis in Y. lipolytica, GGS1 and HMG1, were overexpressed to increase the production of lycopene. All of the genes were also expressed in a Y. lipolytica strain with POX1 to POX6 and GUT2 deleted, which led to an increase in the size of lipid bodies and a further increase in lycopene production. Lycopene is located mainly within lipid bodies, and increased lipid body formation leads to an increase in the lycopene storage capacity of Y. lipolytica. Growth-limiting conditions increase the specific lycopene content. Finally, a yield of 16 mg g(-1) (dry cell weight) was reached in fed-batch cultures, which is the highest value reported so far for a eukaryotic host.