The T cell dependence of B cell differentiation induced by pokeweed mitogen.
The T cell dependence of B cell differentiation induced by pokeweed mitogen.
复制标题
商陆有丝分裂原诱导的 B 细胞分化的 T 细胞依赖性。
DOI：
10.4049/jimmunol.117.5_part_1.1538
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发表时间：
1976
影响因子：
4.4
通讯作者：
A. Lawton
中科院分区：
文献类型：
--
作者：
R. Keightley;M. Cooper;A. Lawton
人外周血B淋巴细胞对脉宽调制的体外浆细胞反应依赖于T淋巴细胞的存在。单独用脉宽调制培养的B淋巴细胞不能增殖或分化为浆细胞。在培养前将T淋巴细胞加回B淋巴细胞后，形成的IgM、Ig G和Ig A三类浆细胞数与回流的T淋巴细胞数成比例增加。外周血和成人胸腺来源的同种异体T细胞均可有效替代自体T细胞。通过比较相同数量的自体和同种异体T细胞培养的B淋巴细胞的反应，发现来自不同正常供者的浆细胞反应量的显著差异与其T细胞的质量有关；来自高应答者的T细胞显著促进来自低应答者的B细胞的浆细胞分化，反之亦然。这种T细胞的“辅助功能”是抗辐射的，是胸腺细胞和外周T细胞的特性。
The in vitro plasma cell response of human peripheral blood B lymphocytes to PWM was found to be dependent on the presence of T lymphocytes. B lymphocytes cultured alone with PWM did not respond with proliferation or differentiation into plasma cells. When T lymphocytes were added back to B lymphocytes before culture, the number of plasma cells of the IgM, IgG and IgA classes formed increased in proportion to the number of T lymphocytes returned. Allogeneic T lymphocytes from either peripheral blood or adult thymus could effectively substitute for autologous T lymphocytes. By comparing responses of B lymphocytes cultured with equal numbers of autologous or allogeneic T cells, it was found that the considerable variation in the magnitude of the plasma cell response from different normal donors was related to a quality of their T cells; T cells from high responders substantially enhanced plasma cell differentiation of B cells from low responders and vice versa. This T cell "helper function" was irradiation-resistant and was a property of thymocytes as well as peripheral T cells.