Feeding live cultures of Enterococcus faecium and Saccharomyces cerevisiae induces an inflammatory response in feedlot steers
Feeding live cultures of Enterococcus faecium and Saccharomyces cerevisiae induces an inflammatory response in feedlot steers
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DOI：
10.2527/jas.2006-216
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发表时间：
2007-01-01
影响因子：
3.3
通讯作者：
Ametaj, B. N.
中科院分区：
文献类型：
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作者：
Emmanuel, D. G. V.;Jafari, A.;Ametaj, B. N.
本研究通过两项试验，研究了单独或联合饲喂产乳酸细菌屎肠球菌EF212对饲养场母猪急性期反应介质的影响。采用2个拉丁方阵，每个方阵内4头牛，2个周期的交叉设计，研究8头有瘘管的牛单独使用粪肠杆菌或与酵母联合使用的效果。每期3周，预试期10 d，测量期11 d。试验饲粮中蒸熟大麦含量为87%，大麦青贮8%，添加5%（干物质基础）。在实验1中，对照处理为产乳酸细菌粪肠杆菌（6 × 10(10) cfu/d）。在实验2中，对照处理为粪肠杆菌（6 × 10(10) cfu/d）和酿酒葡萄球菌（6 × 10(10) cfu/d）。在饲喂时，将细菌和酵母添加物与碳酸钙混合，在饲粮中添加6 × 10(10) cfu/d，每天1次（10 g/d）。饲喂对照日粮的阉牛仅饲喂载体（10 g/d）。于各组第17、21天颈静脉采血，测定血清淀粉样蛋白A （SAA）、脂多糖结合蛋白（LBP）、珠蛋白、α(1)-酸性糖蛋白（α 1- agp）含量。与对照组相比，添加粪肠杆菌对血浆中SAA、LBP、触珠蛋白或α (1)-AGP的浓度没有影响。饲喂粪肠杆菌和酵母菌可提高血清SAA、LBP和触珠蛋白浓度（P = 0.02），但对血浆α (1)-AGP无影响。综上所述，单独口服补充粪肠杆菌对所测急性期反应的介质没有影响，而饲喂粪肠杆菌和酵母会引起饲喂高谷物饲料的饲养场阉牛的炎症反应。需要进一步的研究来确定粪肠杆菌和酵母刺激饲养场牛急性期蛋白产生的机制。
Two experiments were conducted to investigate the effects of oral supplementation of the lactic-acid-producing bacterium Enterococcus faecium EF212 alone or in combination with Saccharomyces cerevisiae (yeast) on mediators of the acute phase response in feedlot steers. Eight fistulated steers were used to study the effects of E. faecium alone or with yeast in a crossover design with 2 Latin squares, 4 steers within each square, and 2 periods. The length of each period was 3 wk, with a 10-d adaptation and an 11-d measurement period. The experimental diet contained 87% steam-rolled barley, 8% whole-crop barley silage, and 5% supplement (DM basis). In Exp. 1, treatments were control vs. the lactic-acid-producing bacterium E. faecium (6 x 10(10) cfu/d). In Exp. 2, treatments were control vs. E. faecium (6 x 10(10) cfu/d) and S. cerevisiae (6 x 10(10) cfu/d). The bacteria and yeast supplements were blended with calcium carbonate to supply 6 x 10(10) cfu/d when top-dressed into the diet once daily at the time of feeding (10 g/d). Steers fed the control diet received only carrier (10 g/d). Blood samples were collected from the jugular vein on d17 and 21 of each period, and serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), haptoglobin, and alpha(1)-acid glycoprotein (alpha 1-AGP) were measured. Supplementation of feed with E. faecium had no effect on concentrations of SAA, LBP, haptoglobin, or alpha(1)-AGP in plasma compared with those of controls. However, feeding E. faecium and yeast increased (P = 0.02) plasma concentrations of SAA, LBP, and haptoglobin but had no effect on plasma alpha(1)-AGP. In conclusion, oral supplementation of E. faecium alone had no effect on the mediators of the acute phase response that were measured, whereas feeding of E. faecium and yeast induced an inflammatory response in feedlot steers fed high-grain diets. Further research is warranted to determine the mechanism(s) by which E. faecium and yeast stimulated production of acute phase proteins in feedlot steers.