EXPERIMENTALLY SYNTHESIZED PLANT CHIMERAS .1. INVITRO RECOVERY OF NICOTIANA-TABACUM-L CHIMERAS FROM MIXED CALLUS-CULTURES
EXPERIMENTALLY SYNTHESIZED PLANT CHIMERAS .1. INVITRO RECOVERY OF NICOTIANA-TABACUM-L CHIMERAS FROM MIXED CALLUS-CULTURES
复制标题
DOI：
10.1093/oxfordjournals.aob.a086820
复制
发表时间：
1984-01-01
期刊：
影响因子：
4.2
通讯作者：
GOUIN, FR
中科院分区：
文献类型：
--
作者：
MARCOTRIGIANO, M;GOUIN, FR
进行实验以开发通过利用体外技术在已知基因型的植物之间合成嵌合体的技术。从白化病和绿色子叶、下胚轴、愈伤组织培养物和细胞悬浮液中诱导愈伤组织，获得由烟草绿色和白化病细胞组成的嵌合愈伤组织。最有效的混合基因型发生时，愈伤组织来自混合过滤的细胞悬浮液。芽再生后，嵌合体愈伤组织产生1317非嵌合体和4嵌合体植物。嵌合体可能是实验过程的结果，也可能是自发的染色体异常，因为某些白化病对照植物的叶子偶尔会产生小的绿色细胞岛。提出了恢复高比例的非嵌合芽的说明。
Experiments were conducted to develop techniques for synthesizing chimeras between plants of known genotype by utilizing in vitro techniques. Chimeral calli composed of green and albino tobacco cells were obtained by initiating callus tissue from mixtures of albino and green cotyledons, hypocotyls, callus cultures and cell suspensions. The most effective mixing of genotypes occurred when callus was derived from mixed filtered cell suspensions. Upon shoot regeneration, chimeral calli yielded 1317 non-chimeral and 4 chimeral plants. Chimeras may have arisen as a result of experimental procedures or possibly from spontaneous chromosomal abnormalities since leaves of some albino control plants occasionally produced small green islands of cells. Explanations for the recovery of a high percentage of non-chimeral shoots are presented.