Arbuscular mycorrhizal fungi alter root and foliar responses to fissure-induced root damage stress
Arbuscular mycorrhizal fungi alter root and foliar responses to fissure-induced root damage stress
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DOI：
10.1016/j.ecolind.2021.107800
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发表时间：
2021-08
影响因子：
6.9
通讯作者：
Jian Zhang;Y. Bi;Ziheng Song;Li-Quan Xiao;P. Christie
中科院分区：
文献类型：
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作者：
Jian Zhang;Y. Bi;Ziheng Song;Li-Quan Xiao;P. Christie
煤矿开采引起的地面沉降会产生大量的地表地裂缝，并对周围的植物根系造成危害。利用室内模拟根系损伤装置，对玉米根系生长进行了真实的实时观测，研究了根系和叶片对裂缝诱导的根系损伤胁迫的形态学响应，以及丛枝菌根真菌（AMF）对根系损伤的影响。首次观察到了根系受损的菌根和对照植物的根和叶生长以及激素变化的时间序列。地裂缝后1周，根系和叶片中吲哚乙酸（IAA）和细胞分裂素（CTK）含量显著下降。脱落酸（阿坝）含量增加，根和叶的生长受到抑制。丛枝菌根真菌（AMF）扭转了这些趋势，并显着增加了根尖和细根的百分比与未接种的对照组相比。地裂缝后6周，根和叶中的IAA和CTK水平显著下降，而阿坝水平保持不变。接种AMF后，IAA和CTK含量均显著增加，阿坝含量显著降低。根系的空间分布除受激素变化的影响外，还受裂隙的影响。收获期接种AMF可缓解地裂缝对玉米生长的负面影响，增加地上部和根系生物量。结果表明，AMF对根系伤害胁迫的缓解作用可能与调节激素水平，增加细根比例，有利于根系和叶片的生长，增强植物对环境胁迫的抵抗力有关。此外，变量分布分析表明，AMF的改良效果受根系损伤程度的影响。
Ground subsidence caused by coal mining can produce large amounts of surface ground fissures which may damage the surrounding plant roots. Here, a device was used that simulates root damage and we observed the growth of maize roots in real time, the morphological responses of roots and leaves to fissure-induced root damage stress, and the effects of arbuscular mycorrhizal fungi (AMF). Time series of root and leaf growth and hormonal changes in mycorrhizal and control plants with damaged root systems were observed for the first time. Indole-3-acetic acid (IAA) and cytokinin (CTK) levels in roots and leaves decreased significantly one week after ground fissuring. Abscisic acid (ABA) levels increased and the growth of roots and leaves was inhibited. Arbuscular mycorrhizal fungi (AMF) reversed these trends and significantly increased the percentages of root tips and fine roots compared with uninoculated controls. IAA and CTK levels declined significantly in roots and leaves six weeks after ground fissuring but ABA levels remained unchanged. AMF inoculation significantly increased both IAA and CTK levels but decreased ABA levels. The spatial distribution of roots was affected by fissures in addition to the changes in hormones. The negative impacts of ground fissures on maize growth were alleviated by AMF inoculation at harvest with increased shoot and root biomass. The results suggest that the ameliorative effects of AMF on root damage stress may be related to the regulation of hormone levels and an increase in the percentage of fine roots which was conducive to the growth of roots and leaves and increased plant resistance to environmental stress. In addition, analysis of variable distributions shows that the ameliorative effects of AMF were affected by the degree of root damage.