Dissection of Pharmacological Mechanism of Chinese Herbal Medicine Yihuo Huatan Formula on Chronic Obstructive Pulmonary Disease: A Systems Pharmacology-Based Study
Dissection of Pharmacological Mechanism of Chinese Herbal Medicine Yihuo Huatan Formula on Chronic Obstructive Pulmonary Disease: A Systems Pharmacology-Based Study
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DOI：
10.1038/s41598-019-50064-9
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发表时间：
2019-09
影响因子：
4.6
通讯作者：
Xia-Wei Zhang;Wei Liu;Hongli Jiang;B. Mao
中科院分区：
文献类型：
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作者：
Xia-Wei Zhang;Wei Liu;Hongli Jiang;B. Mao
慢性阻塞性肺疾病（COPD）是最常见的呼吸系统疾病之一。益活化痰方（YHF）作为一种中药，经过多年的实践，已被证实对稳定期慢性阻塞性肺疾病（COPD）有较好的疗效。然而，其工作机制尚不清楚。我们试图通过基于系统药理学的方法系统地解读YHF治疗稳定期COPD的机制，该方法整合了药代动力学筛选、靶标预测、网络分析、GO和KEGG富集分析。首先，将15种中药成分中的1267种化学物质纳入YHF化学数据库。通过药代动力学评价筛选出180个潜在活性分子。预测了258个活性分子靶点，并从中筛选出84个进行进一步分析。最后，网络分析和GO和KEGG富集方法提示，YHF通过调节多种靶点和途径，具有减轻气道炎症、减少粘液分泌、维持免疫稳态和改善COPD合并症的治疗作用。基于系统药理学的方法有助于从整体角度了解YHF在稳定型COPD中的潜在作用机制，并为系统揭示CHM的作用机制提供范例。
Chronic obstructive pulmonary disease (COPD) is one of the most common respiratory diseases. Yihuo Huatan Formula (YHF), as a proven Chinese Herbal Medicine (CHM), has been verified to be effective in the treatment of stable COPD through years’ of practice. Nevertheless, its working mechanism is still unclear. We sought to systematically decipher the mechanism of YHF for treating stable COPD using systems pharmacology-based method that integrates pharmacokinetic screening, target prediction, network analyses, GO and KEGG enrichment analyses. Firstly, a total of 1267 chemicals out of 15 herbal components were included in YHF chemical database. Among them, 180 potential active molecules were screened out through pharmacokinetic evaluation. Then 258 targets of the active molecules were predicted, of which 84 were chosen for further analyses. Finally, the network analyses and GO and KEGG enrichment methods suggested a therapeutic effect of YHF on the alleviation of airway inflammation, decrease of mucus secretion, maintenance of immune homeostasis and benefit of COPD comorbidities, by regulating multiple targets and pathways. The systems pharmacology-based approach helps to understand the underlying working mechanism of YHF in stable COPD from a holistic perspective, and offers an exemplification for systematically uncovering the action mechanisms of CHM.