Alignment and calibration of a focal neurotransmitter uncaging system.
Alignment and calibration of a focal neurotransmitter uncaging system.
复制标题
焦点神经递质释放系统的对准和校准。
DOI：
10.1038/nprot.2006.124
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发表时间：
2006
期刊：
影响因子：
14.8
通讯作者：
Wang,SamuelS-H
中科院分区：
文献类型：
--
作者：
Sarkisov,DmitryV;Wang,SamuelS-H
笼状化合物的光解是研究亚细胞生理功能的有力工具。在这里，我们描述了焦点解锁系统的对齐和校准协议。我们还报告了方便定量校准解笼的程序。使用这些方法，我们可以实现光解的亚微米横向分辨率，并探测棘（神经元最小的信号传导室）的生物功能。最初，整个对准过程需要 4-6 小时才能完成；系统的定期微调需要1-2小时。
Photolysis of caged compounds is a powerful tool for studying subcellular physiological functions. Here we describe protocols for the alignment and calibration of a focal uncaging system. We also report procedures for convenient quantitative calibration of uncaging. Using these methods, we can achieve submicron lateral resolution of photolysis and probe biological function in spines, the smallest signaling compartments of neurons. Initially, the entire alignment procedure takes 4–6 h to perform; periodic fine-tuning of the system takes 1–2 h.