An established hybridoma clone producing a monoclonal antibody against Vibrio anguillarum
An established hybridoma clone producing a monoclonal antibody against Vibrio anguillarum
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DOI：
10.1271/bbb.61.1939
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发表时间：
1997-11-01
影响因子：
1.6
通讯作者：
Hara, S
中科院分区：
文献类型：
--
作者：
Ikura, K;Kodama, S;Hara, S
鳗弧菌是弧菌病的病原微生物，弧菌病是一种在各种鱼类中发现的传染病。建立了一株能产生抗鳗弧菌单抗的小鼠杂交瘤克隆C5。通过酶联免疫吸附试验和细胞免疫沉淀实验，证实了C5抗体与鳗弧菌的特异性反应。Western blotting分析表明，C5抗体识别从含有洗涤剂的细胞中提取的高分子量物质。
Vibrio anguillarum is a pathogenic microorganism of vibriosis, an infectious disease found in various fish species. A mouse hybridoma clone, named C5, that produced a monoclonal antibody to V. anguillarum was established. The specific reaction of C5 antibody with V. anguillarum was confirmed by the pre-adsorption effect of the V. anguillarum cells in ELISA and a cell immunoprecipitation experiment. Western blotting analysis indicated that the C5 antibody recognized a high molecular weight substance extracted from cells with detergents.