MONOCLONAL-ANTIBODIES SPECIFIC TO NATIVE MURINE T-CELL RECEPTOR-GAMMA-DELTA - ANALYSIS OF GAMMA-DELTA-T-CELLS DURING THYMIC ONTOGENY AND IN PERIPHERAL LYMPHOID ORGANS
MONOCLONAL-ANTIBODIES SPECIFIC TO NATIVE MURINE T-CELL RECEPTOR-GAMMA-DELTA - ANALYSIS OF GAMMA-DELTA-T-CELLS DURING THYMIC ONTOGENY AND IN PERIPHERAL LYMPHOID ORGANS
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DOI：
10.1073/pnas.86.13.5094
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发表时间：
1989-07-01
影响因子：
11.1
通讯作者：
TONEGAWA, S
中科院分区：
文献类型：
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作者：
ITOHARA, S;NAKANISHI, N;TONEGAWA, S
三种仓鼠单克隆抗体（mAbs），全部识别存在于鼠T细胞抗原受体（TCR）γ的天然形式上的不同表位。δ的亚单位，已经产生。mAb 3A 10对全鼠TCR γ具有特异性。δ，识别C δ。恒定区决定簇mAb 8D 6对表达V γ 4和V δ 5编码的γ的T细胞亚群具有特异性。mAb 5C 10是克隆型的。使用这些和其它针对多种T细胞表面标志物的mAb，我们定量和表征了γ。δ的通过流式细胞术，T细胞存在于发育中的胸腺以及常规淋巴器官中。这些研究揭示了（i）许多γ。δ的胸腺细胞和外周T细胞携带CD 4和/或CD 8分子，[ii] T细胞携带两者α。β的和γ。δ的TCR是稀缺的，和（iii）携带TCR γ的胸腺细胞亚群。δ的由V γ的不同组合编码。和V δ。基因片段在个体发育中呈波浪状出现。
Three hamster monoclonal antibodies (mAbs), all recognizing different epitopes present on the native form of the murine T-cell antigen receptor (TCR) .gamma..delta. subunits, have been generated. mAb 3A10 is specific to a pan-murine TCR .gamma..delta., recognizing a C.delta. constant region determinant. mAb 8D6 is specific to a subset of T cells expressing V.gamma.4- and V.delta.5-encoded .gamma..delta.TCR, and mAb 5C10 is clonotypic. Using these and other mAbs directed against a variety of T-cell surface markers, we quantitated and characterized .gamma..delta. T cells present in developing thymuses as well as in the conventional lymphatic organs by flow cytometry. These studies revealed that (i) many .gamma..delta. thymocytes and peripheral T cells bear CD4 and/or CD8 molecules, [ii) T cells bearing both .alpha..beta. and .gamma..delta. TCRs are scarce, and (iii) thymocyte subsets bearing TCR .gamma..delta. encoded by different combinations of V.gamma. and V.delta. gene segments appear in waves during ontogeny.