Pseudomonas yangonensis sp. nov., isolated from wound samples of patients in a hospital in Myanmar
Pseudomonas yangonensis sp. nov., isolated from wound samples of patients in a hospital in Myanmar
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DOI：
10.1099/ijsem.0.004181
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发表时间：
2020-01-01
影响因子：
2.8
通讯作者：
Tin, Htay Htay
中科院分区：
文献类型：
--
作者：
Tohya, Mari;Watanabe, Shin;Tin, Htay Htay
从缅甸仰光3名住院患者的伤口样本中分离出革兰氏阴性、需氧、棒状、非孢子形成细菌，命名为MY50(T)、MY63和MY101。菌株MY50T、MY63和MY101在温度4-44℃、NaCl含量1.0- 7.0% （w/v）、pH 6.0-9.5的培养基中生长。基于16S rRNA基因和全基因组序列的系统发育分析表明，这些菌株属于假单胞菌属，属于油梨假单胞菌属，与古莞假单胞菌和mendocina假单胞菌位置相近。利用平均核苷酸身份和数字DNA-DNA杂交分析进行全基因组比较，证实菌株MY50(T)， MY63和MY101是同一菌株，它们是P. oleovorans组中的不同物种。表型鉴定结果表明，利用对羟基苯基乙酸、甘油、l-焦谷氨酸和奎宁酸可以将这些菌株与其他种类的p. oleovorans群区分开来。这些遗传和表型特征表明，它们应该被归类为代表一个新种，并被命名为Pseudomonas yangonensis sp. 11 .该型菌株为MY50(T) (=LMG 31602(T),=JCM 33396(T))， DNA G+C含量为62.82 mol%。
Strains of a Gram-negative, aerobic, rod-shaped, non- spore-forming bacterium, designated MY50(T), MY63 and MY101, were isolated from wound samples of three hospitalized patients in Yangon, Myanmar. Strains MY50T, MY63 and MY101 grew at temperatures of 4-44 degrees C, in media containing 1.0-7.0 % (w/v) NaCl and at pH 6.0-9.5. Phylogenetic analysis based on 16S rRNA gene and whole genome sequences showed that these strains belonged to the genus Pseudomonas and were part of the Pseudomonas oleovorans group and located close to Pseudomonas guguanensis and Pseudomonas mendocina. Whole-genome comparisons, using average nucleotide identity and digital DNA-DNA hybridization analyses, confirmed that strains MY50(T), MY63 and MY101 were the same strain and they were a distinct species in the P. oleovorans group. Results of phenotypic charac-terization tests demonstrated that utilization of p- hydroxy-phenylacetic acid, glycerol, l- pyroglutamic acid and quinic acid could distinguish these strains from other species of the P. oleovorans group. These genetic and phenotypic characteristics suggest that they should be classified as representing a novel species, under the proposed name Pseudomonas yangonensis sp. nov. The type strain is MY50(T) (=LMG 31602(T),=JCM 33396(T)), with a DNA G+C content of 62.82 mol%.