Hypoxia regulates PGE2 release and EP1 receptor expression in osteoblastic cells
Hypoxia regulates PGE2 release and EP1 receptor expression in osteoblastic cells
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缺氧调节成骨细胞中 PGE2 释放和 EP1 受体表达
DOI：
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发表时间：
2007
影响因子：
5.6
通讯作者：
Clare E. Yellowley
中科院分区：
文献类型：
--
作者：
Christina M. Lee;D. Genetos;Z. You;Clare E. Yellowley
在骨折和骨骼卸载过程中发生的局部O2张力的变化可能会刺激局部骨细胞活性，并最终调节骨的维持和修复。骨细胞感知和响应O2张力变化的机制尚不清楚。在这项研究中，我们研究了低氧对MC 3 T3-E1成骨细胞中缺氧反应元件（HRE）激活、前列腺素E2（PGE 2）产生、PGE 2受体（EP）表达和增殖的影响。将细胞在2%O2（认为是缺氧的）、5%O2（在体内正常O2张力范围内）或21%O2（通常用于细胞培养）中培养长达72小时。与21%O2下生长的细胞相比，2%O2下培养的细胞显示HRE活化，PGE 2释放增加，EP 1表达增加，细胞增殖减少。同样，在5%O2中培养的细胞表现出EP 1表达增加和增殖减少的趋势，但HRE没有活化或PGE 2水平增加。O2张力对EP 2、EP 3和EP 4的表达无影响。与21% O2相比，在5% O2下生长的细胞中观察到的EP受体谱差异表明，在常规细胞培养条件下，骨细胞表型可能会改变。此外，我们的数据表明，骨细胞中缺氧依赖性PGE 2的产生和EP 1的表达可能在骨重建和受损或受损骨区域的修复中发挥作用，其中O2张力较低。J.细胞。212：182-188，2007。© 2007 Wiley利斯公司
Changes in regional O2 tension that occur during fracture and skeletal unloading may stimulate local bone cell activity and ultimately regulate bone maintenance and repair. The mechanisms by which bone cells sense and respond to changes in O2 tension are unclear. In this study we investigated the effects of low O2 on activation of the hypoxia response element (HRE), prostaglandin E2 (PGE2) production, PGE2 receptor (EP) expression and proliferation in MC3T3‐E1 osteoblastic cells. Cells were cultured for up to 72 h in 2% O2 (considered hypoxic), 5% O2 (in the range of normal O2 tension in vivo) or 21% O2 (commonly used for cell culture). Cells cultured in 2% O2 showed activation of the HRE, increased PGE2 release, increased EP1 expression, and reduced cell proliferation compared to cells grown at 21% O2. Similarly, cells cultured in 5% O2 showed increased expression of EP1 and a trend toward a decrease in proliferation, but no activation of the HRE or increase in PGE2 levels. Expression of EP2, EP3 and EP4 were not affected by O2 tension. The differences in EP receptor profile observed in cells grown at 5% compared to 21% O2 suggest that bone cell phenotype may be altered under routine cell culture conditions. Furthermore, our data suggest that hypoxia‐dependent PGE2 production and EP1 expression in bone cells may play a role in bone remodeling and repair in regions of compromised or damaged bone, where O2 tension is low. J. Cell. Physiol. 212: 182–188, 2007. © 2007 Wiley‐Liss, Inc.
DOI：
10.1152/ajpcell.1999.277.3.c598
发表时间：
1999-09-01
影响因子：
5.5
作者：
Dodd, JS;Raleigh, JA;Gross, TS
通讯作者：
Gross, TS
小鼠前列腺素 EP2 受体的敲除会损害体外破骨细胞生成。
影响因子：
4.8
作者：
Li,X;Okada,Y;Pilbeam,CC;Lorenzo,JA;Kennedy,CR;Breyer,RM;Raisz,LG
通讯作者：
Raisz,LG