Highly sensitive and selective colorimetric sensing of antibiotics in milk.
Highly sensitive and selective colorimetric sensing of antibiotics in milk.
复制标题
DOI：
10.1016/j.aca.2013.03.059
复制
发表时间：
2013-05
影响因子：
6.2
通讯作者：
Xiaofang Zhang;Yang Zhang;Hong Zhao;Yujian He;Xiangjun Li;Zhuo-bin Yuan
中科院分区：
文献类型：
--
作者：
Xiaofang Zhang;Yang Zhang;Hong Zhao;Yujian He;Xiangjun Li;Zhuo-bin Yuan
食品中的抗生素残留对人体危害很大。抗生素残留的测定很大程度上取决于是否有足够的分析技术。目前迫切需要对食品中的抗生素进行现场实时检测。在这项工作中，提出了一种使用邻苯二酚紫（PCV）作为还原剂一步合成金纳米颗粒（AuNP）的新方法。在AuNPs的形成过程中，实现了单硫酸卡那霉素（KA）、硫酸新霉素（NE）、硫酸链霉素（ST）和硫酸博来霉素（BL）四种抗生素的高灵敏和选择性比色检测。 PCV具有OH基团，而这些抗生素具有OH、NH2、NH基团，因此PCV与这些抗生素之间可能存在一些特殊的氢键相互作用。因此，KA、NE、ST和BL的存在会影响AuNPs的合成，从而使AuNPs的颜色和状态发生变化，这可以用肉眼或紫外可见分光光度计观察到。结果表明，在1.0×10−8～5.0×10−7M和5.0×10−7～5.5×10−5M范围内，A670与KA浓度的对数呈线性关系。 KA的检出限为1.0×10−9M（S/N=3）。 1.0×10−5M苯丙氨酸、丙氨酸、甘油、葡萄糖、Mg2+、Ca2+、Na+、K+、CO32−、SO42−、NO3−、Cl−、Br−等共存物质不影响1.0×10−7M抗生素的测定。特别是，所提出的方法可以成功应用于预处理液态奶制品中抗生素的检测。
Antibiotics residues in foods are very harmful to human beings. Determination of antibiotics residues relies largely on the availability of adequate analytical techniques. Currently, there is an urgent need for on site and real time detection of antibiotics in food. In this work, a novel one step synthesis of gold nanoparticles (AuNPs) was proposed using pyrocatechol violet (PCV) as a reducer agent. Highly sensitive and selective colorimetric detection of four antibiotics kanamycin mono sulfate (KA), neomycin sulfate (NE), streptomycin sulfate (ST) and bleomycin sulfate (BL) was realized during the formation of AuNPs. PCV has OH groups and these antibiotics have OH, NH2, NH groups, so there may be some special hydrogen-bonding interactions between PCV and these antibiotics. Therefore, the presence of KA, NE, ST and BL would influence the synthesis of AuNPs, then the color and state of AuNPs would change, which could be observed with the naked eye or a UV–vis spectrophotometer. Results showed that A670was linear with the logarithm of KA concentration in the range from 1.0×10−8to 5.0×10−7M and 5.0×10−7to 5.5×10−5M. The detection limit of KA was 1.0×10−9M (S/N=3). The coexisting substances including 1.0×10−5M phenylalanine, alanine, glycerol, glucose, Mg2+, Ca2+, Na+, K+, CO32−, SO42−, NO3−, Cl−and Br−did not affect the determination of 1.0×10−7M antibiotics. In particular, the proposed method could be applied successfully to the detection of antibiotics in the pretreated liquid milk products.