Silencing of Nogo-A in rat oligodendrocyte cultures enhances process branching
Silencing of Nogo-A in rat oligodendrocyte cultures enhances process branching
复制标题
大鼠少突胶质细胞培养物中 Nogo-A 的沉默增强了过程分支
DOI：
10.1016/j.neulet.2011.05.026
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发表时间：
2011-07
期刊：
影响因子：
--
通讯作者：
赵湘辉
中科院分区：
文献类型：
--
作者：
赵湘辉
髓鞘相关蛋白Nogo-A是一种众所周知的轴突再生和代偿可塑性抑制剂，但内源性Nogo-A在少突胶质细胞分化中的功能尚不清楚。随着少突胶质细胞的成熟，其突起分支并最终形成包裹靶轴突的薄片。本研究检测了Nogo-A水平降低对少突胶质细胞发育的影响。在这些细胞中siRNA介导的Nogo-A沉默不改变其通过BrdU掺入测定鉴定的增殖速率，也不改变通过qRT-PCR和免疫染色鉴定的阶段特异性少突胶质细胞标记物的表达。Sholl分析表明，敲除Nogo-A的表达显著增加了过程分支的复杂性。目前的研究结果表明，Nogo-A在少突胶质细胞过程生长中维持限制性分支表型的新作用，这是髓鞘膜片形成和髓鞘形成的关键步骤。
The myelin-associated protein Nogo-A is a well-known inhibitor for axonal regeneration and compensatory plasticity, yet functions of endogenous Nogo-A in oligodendrocyte differentiation are not as clear. As oligodendrocyte matures, its processes branch and eventually form lamellae that ensheath target axons. The present study examined the effects of decreased levels of Nogo-A on the development of oligodendrocytes. The siRNA mediated Nogo-A silencing in these cells did not change their proliferation rates identified by BrdU incorporation assay and neither the expression of stage specific oligodendrocyte makers as identified by qRT-PCR and immunostaining. But knockdown the expression of Nogo-A significantly enhances the process branching complexity by Sholl analysis. Current results suggest a novel role for Nogo-A in maintaining a restricted branching phenotype in oligodendrocytes process outgrowth, which is a key step towards myelin membrane sheet formation and myelination.
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DOI：
10.1083/jcb.200411047
发表时间：
2005-08-15
期刊：
The Journal of cell biology
影响因子：
--
作者：
Lee J;Gravel M;Zhang R;Thibault P;Braun PE
通讯作者：
Braun PE
影响因子：
5.2
作者：
B. Zörner;M. Schwab
通讯作者：
B. Zörner;M. Schwab
影响因子：
4.7
作者：
S. Rajasekharan;Jenea M Bin;J. Antel;Timothy E. Kennedy
通讯作者：
S. Rajasekharan;Jenea M Bin;J. Antel;Timothy E. Kennedy
影响因子：
11.2
作者：
Yang, Yuhong;Liu, Yue;Wei, Ping;Peng, Haiyan;Winger, Ryan;Hussain, Rehana Z.;Ben, Li-Hong;Cravens, Petra D.;Gocke, Anne R.;Puttaparthi, Krishna;Racke, Michael K.;McTigue, Dana M.;Lovett-Racke, Amy E.
通讯作者：
Lovett-Racke, Amy E.
影响因子：
3.7
作者：
Meur, Ana Mingorance-Le;Zheng, Binhai;del Rio, Jose A.
通讯作者：
del Rio, Jose A.