CLONING AND GROWTH OF MULTIPOTENTIAL NEURAL PRECURSORS - REQUIREMENTS FOR PROLIFERATION AND DIFFERENTIATION
CLONING AND GROWTH OF MULTIPOTENTIAL NEURAL PRECURSORS - REQUIREMENTS FOR PROLIFERATION AND DIFFERENTIATION
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DOI：
10.1016/0896-6273(93)90316-j
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发表时间：
1993-02-01
期刊：
影响因子：
16.2
通讯作者：
BARTLETT, PF
中科院分区：
文献类型：
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作者：
KILPATRICK, TJ;BARTLETT, PF
利用胚胎第10天的小鼠神经上皮细胞，从端脑和中脑分离，评估了内在承诺和表观遗传影响对成熟神经细胞表型发育的重要性。用成纤维细胞生长因子生成两种类型的克隆：a型克隆由大的无定形细胞组成，b型克隆含有上皮样细胞。在许多b型克隆中，产生了大量的前体细胞。24%的b型克隆含有少量神经元，59%含有神经元的克隆也含有星形胶质细胞，表明这种克隆类型来源于双电位前体细胞。从永生化星形胶质样细胞系中提取的前体细胞用条件培养基培养，可以增强神经元的分化。这些结果表明，神经上皮前体细胞的增殖和分化具有离散的表观遗传要求。
The importance of intrinsic commitments and epigenetic influence to the development of mature neural cell phenotypes was assessed using embryonic day 10 murine neuroepithelial cells, isolated from telencephalon and mesencephalon. Two types of clones were generated with fibroblast growth factor: type-A clones consisted of large, amorphous cells, and type-B clones contained epithelial-like cells. In many type-B clones, very large numbers of precursor cells were produced. Twenty-four percent of type-B clones contained small numbers of neurons, and 59% of clones containing neurons also contained astrocytes, indicating that this clonal type was derived from a bipotential precursor cell. Neuronal differentiation was enhanced by culturing precursor cells with conditioned medium derived from an immortalized astroglial-like cell line. These results indicate that neuroepithelial precursors have discrete epigenetic requirements for their proliferation and differentiation.