The ecdysone regulatory pathway controls wing morphogenesis and integrin expression during Drosophila metamorphosis
The ecdysone regulatory pathway controls wing morphogenesis and integrin expression during Drosophila metamorphosis
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DOI：
10.1006/dbio.2000.9650
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发表时间：
2000-04-15
影响因子：
2.7
通讯作者：
Thummel, CS
中科院分区：
文献类型：
--
作者：
D'Avino, PP;Thummel, CS
果蝇成虫盘在胚胎和幼虫发育过程中被指定和图案化，导致每个细胞在成虫中获得特定的命运。然而，成虫组织的形态发生和分化直到变态才发生，此时类固醇激素蜕皮激素的脉冲指导这些复杂的形态发生反应。在本文中，我们集中在蜕皮激素的作用，在调节成虫翅发育变态。我们发现，突变的EcR蜕皮激素受体基因和弯曲的腿（cro 1），蜕皮激素诱导基因编码的锌指蛋白家族，导致类似的缺陷翅形态和细胞粘附，表明蜕皮激素在这些形态发生反应的作用。我们还表明，cro 1和EcR突变与编码整合素亚单位的基因突变相互作用，整合素亚单位是一个α β异二聚体细胞表面受体家族，在许多生物体中介导细胞粘附。在培养的幼虫器官中，α-整联蛋白转录受蜕皮激素调节，并且整联蛋白表达的时间模式的一些变化与变态期间蜕皮激素滴度谱相关。α-和β-整联蛋白亚基的转录也在cro 1和EcR突变体中改变，表明整联蛋白表达依赖于cro 1和EcR功能。最后，我们描述了一个新的亚型突变的EcR这表明，不同的EcR亚型可以指导成人附属器的发展。本研究为蜕皮激素通过调控整联蛋白在变态过程中的表达来控制翅的形态发生和细胞粘附提供了证据。我们还提出，蜕皮激素调节整合素的表达可能被广泛用于控制成人发育的多个方面。(C)北京大学出版社.
Drosophila imaginal discs are specified and patterned during embryonic and larval development, resulting in each cell acquiring a specific fate in the adult ny. Morphogenesis and differentiation of imaginal tissues, however, does not occur until metamorphosis, when pulses of the steroid hormone ecdysone direct these complex morphogenetic responses. In this paper, we focus on the role of ecdysone in regulating adult wing development during metamorphosis. We show that mutations in the EcR ecdysone receptor gene and crooked legs (cro1), an ecdysone-inducible gene that encodes a family of zinc finger proteins, cause similar defects in wing morphogenesis and cell adhesion, indicating a role for ecdysone in these morphogenetic responses. We also show that cro1 and EcR mutations interact with mutations in genes encoding integrin subunits-a family of alpha beta heterodimeric cell surface receptors that mediate cell adhesion in many organisms. alpha-Integrin transcription is regulated by ecdysone in cultured larval organs and some changes in the temporal patterns of integrin expression correlate with the ecdysone titer profile during metamorphosis. Transcription of alpha- and beta-integrin subunits is also altered in cro1 and EcR mutants, indicating that integrin expression is dependent upon cro1 and EcR function. Finally, we describe a new hypomorphic mutation in EcR which indicates that different EcR isoforms can direct the development of adult appendages. This study provides evidence that ecdysone controls wing morphogenesis and cell adhesion by regulating integrin expression during metamorphosis. We also propose that ecdysone modulation of integrin expression might be widely used to control multiple aspects of adult development. (C) 2000 Academic Press.