Alleviation of drought stress in the common bean (Phaseolus vulgaris L.) by co-inoculation with Paenibacillus polymyxa and Rhizobium tropici
Alleviation of drought stress in the common bean (Phaseolus vulgaris L.) by co-inoculation with Paenibacillus polymyxa and Rhizobium tropici
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DOI：
10.1016/j.apsoil.2008.04.005
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发表时间：
2008-09-01
影响因子：
4.8
通讯作者：
Chanway, Christopher P.
中科院分区：
文献类型：
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作者：
Figueiredo, Marcia V. B.;Burity, Helio A.;Chanway, Christopher P.
通过温室盆栽试验，研究了根瘤菌单独接种和混合接种对菜豆生长、氮含量、激素水平和结瘤的影响。在三种程度的干旱胁迫下。通过多孔杯控制基质电势(psi(M))连续施加应力。豆类植物cv.在盆栽条件下，采用富营养化土壤，在3种不同的psi(M)下(S-1-7.0psi-1-7.0psi(M)、S-2-70.0 psi-2-70.0和S-3-85kPa.分别用热带根瘤菌(CIAT899)、多粘根瘤菌(DSM 36)和L根瘤菌(CIAT899+DSM36+Loutit)混合接种种子。与单独接种根瘤菌相比，根瘤菌和两株拟青霉共接种菜豆能促进植株生长，提高植株含氮量和结瘤能力。这在我们使用的最负psi(M)(S-3和-85千帕)时尤其明显。干旱胁迫引起了植物激素平衡的变化，包括叶片脱落酸(ABA)含量增加，吲哚乙酸(IAA)和赤霉酸(GA(3))含量略有下降，菜豆叶片玉米素含量急剧下降。水分胁迫下，内源CKs含量下降，可能放大了地上部对ABA含量升高的响应。我们推测，与热带赤霉菌(CIAT899)、多粘菌(DSM 36)和鲁蒂特(L)共接种菜豆，可以减轻干旱胁迫对菜豆的一些负面影响。爱思唯尔出版公司(Elsevier B.V.)
A greenhouse experiment was performed to evaluate the influence of Rhizobium when co-inoculated with each of two Paenibacillus polymyxa strains, singly and in mixture on growth, nitrogen content, phytohormone levels and nodulation of the common bean (Phaseolus vulgaris L.) under three levels of drought stress. Stress was applied continuously by the control of matric potential (psi(m)) through a porous cup. Bean plants cv. Tenderlake were grown in pots with Fluvic Neosol eutrophic soil under three different psi(m) (S-1 -7.0; S-2 -70.0 and S-3 < -85 kPa). The seeds were inoculated with Rhizobium tropici (CIAT 899) and each of P. polymyxa (DSM 36) and P. polymyxa Loutit (L) singly and in mixture (CIAT 899 + DSM36 + Loutit). Co-inoculation of bean with Rhizobium and both Paenibacillus strains resulted increased plant growth, nitrogen content and nodulation compared to inoculation with Rhizobium alone. This was particularly evident at the most negative psi(m) (S-3 < -85 kPa) we used. Drought stress triggered a change in phytohormonal balance, including an increase in leaf abscisic acid (ABA) content, a small decline in indole acetic acid (IAA) and gibberellic acid (GA(3)) and a sharp fall in zeatin content in bean leaves. The content of endogenous Cks decreased under water stress, possibly amplifying the response of shoots to increasing ABA content. We hypothesize that co-inoculation of bean with R. tropici (CIAT 899) and P. polymyxa strains (DSM 36) and Loutit (L) mitigates some of the negative effects of drought stress on bean. Published by Elsevier B.V.