HOMOLOGY OF THE HUMAN INTESTINAL NA+/GLUCOSE AND ESCHERICHIA-COLI NA+/PROLINE COTRANSPORTERS
HOMOLOGY OF THE HUMAN INTESTINAL NA+/GLUCOSE AND ESCHERICHIA-COLI NA+/PROLINE COTRANSPORTERS
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DOI：
10.1073/pnas.86.15.5748
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发表时间：
1989-08-01
影响因子：
11.1
通讯作者：
WRIGHT, EM
中科院分区：
文献类型：
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作者：
HEDIGER, MA;TURK, E;WRIGHT, EM
共转运蛋白负责细胞内有机底物的主动积累。Na+梯度为真核生物摄取大多数底物和一些原核生物摄取少数底物提供驱动力。我们在这里报告的克隆和测序的人肠钠+/葡萄糖协同转运蛋白（SGLT 1），并比较其结构与其他克隆的转运蛋白。在DNA水平和预测的氨基酸和二级结构水平上，人和兔肠道Na+/葡萄糖协同转运蛋白之间的同源性是显而易见的，并且在这些和大肠杆菌Na+/脯氨酸协同转运蛋白（putP）之间发现了显著的同源性。与其他已知蛋白质没有可检测的同源性。我们从这些结果推断，哺乳动物Na+/葡萄糖和原核生物Na+/脯氨酸共转运蛋白共享一个共同的祖先基因。
Cotransport proteins are responsible for the active accumulation of organic substrates in cell. Na+ gradients provide the driving force for uptake of most substrates into eukaryotes and for a few substrates in some prokaryotes. We report here the cloning and sequencing of the human intestinal Na+/glucose cotransporter (SGLT1) and compare its structure with other cloned transporters. At the DNA level and the predicted amino acid and secondary structure levels, close homology is evident between the human and rabbit intestinal Na+/glucose cotransporters, and a significant homology is found between these and the Escherichia coli Na+/proline cotransporter (putP). No homology is detectible with other known proteins. We infer from these results that the mammalian Na+/glucose and prokaryote Na+/proline cotransporters share a common ancestral gene.