Peroxisome-proliferator regulates key enzymes of the tryptophan-NAD+ pathway.
Peroxisome-proliferator regulates key enzymes of the tryptophan-NAD+ pathway.
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过氧化物酶体增殖物调节色氨酸-NAD 途径的关键酶。
DOI：
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发表时间：
1999
影响因子：
3.8
通讯作者：
C. Umezawa
中科院分区：
文献类型：
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作者：
M. Shin;M. Ohnishi;S. Iguchi;K. Sano;C. Umezawa
研究了结构多样的过氧化物酶体增殖物（PPs）对Sprague-Dawley雄性大鼠肝脏中色氨酸(Trp)-NAD+通路两种关键酶活性和NAD+水平的影响。除甲状腺素外，所有PPs均显著升高肝脏NAD+水平，并伴有肝脏肥大。ppps增加了Trp-NAD+通路关键酶之一的喹啉酸磷酸核糖基转移酶（QAPRTase）活性，导致肝脏NAD+显著升高。另一方面，Trp-NAD+通路的另一关键酶α -氨基- β -羧酸酯-epsilon-半醛脱羧酶（acmssdase）被除亚麻酸外的所有PPs均显著抑制，仅轻微抑制。大多数PPs研究了活化的过氧化物酶体标记酶，如棕榈酰辅酶a氧化酶、过氧化氢酶和ppar - α（过氧化物酶体增殖物活化受体α）依赖酶，如苹果酸酶和l-3-甘油磷酸脱氢酶。在添加PPs的培养液中培养的大鼠肝细胞中NAD+也有所增加。这些数据表明，Trp-NAD+通路中关键酶的调节与ppar - α直接或间接相关，因此PPs增加了肝脏NAD+。
Structually diverse peroxisome-proliferators (PPs) were investigated regarding their effects on NAD+ level and two key enzyme activities in the tryptophan (Trp)-NAD+ pathway in the liver of rats (Sprague-Dawley male) fed PP-containing diets freely for 2 weeks. All PPs, except for thyroxine, significantly increased hepatic NAD+ level in concert with hepatic hypertrophy. Activity of quinolinate phosphoribosyltransferase (QAPRTase), one of the key enzymes in the Trp-NAD+ pathway, was increased by the PPs which caused significant increase in the hepatic NAD+. On the other hand, alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase (ACMSDase), another key enzyme in the Trp-NAD+ pathway, was drastically inhibited by all PPs except for linolenic acid, which was only slightly inhibitory. Most PPs investigated activated peroxisomal marker enzymes such as palmitoyl-CoA oxidase, catalase, and PPAR-alpha(peroxisome-proliferator activated receptor-alpha)-dependent enzymes, such as malic enzyme and l-3-glycerophosphate dehydrogenase. NAD+ was also increased in the rat hepatocytes cultured in the medium supplemented with PPs. These data suggested that regulation of the key enzymes in the Trp-NAD+ pathway was associated with PPAR-alpha directly or indirectly, and as a consequence the hepatic NAD+ was increased by PPs.
DOI：
10.1073/pnas.93.18.9443
发表时间：
1996-09-03
影响因子：
11.1
作者：
Miller, CW;Ntambi, JM
通讯作者：
Ntambi, JM
花生四烯酸通过抑制转录和增强 mRNA 更新来下调 3T3-L1 脂肪细胞中的胰岛素依赖性葡萄糖转运蛋白基因 (GLUT4)。
DOI：
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发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Tebbey,PW;McGowan,KM;Stephens,JM;Buttke,TM;Pekala,PH
通讯作者：
Pekala,PH
过氧化物酶体增殖物激活受体 α 与类视黄醇 X 受体 α 的相互作用揭示了一个隐秘的过氧化物酶体增殖物反应元件，该元件与 CYP4A6 启动子中的 ARP-1 结合位点重叠。
DOI：
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发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Palmer,CN;Hsu,MH;Muerhoff,AS;Griffin,KJ;Johnson,EF
通讯作者：
Johnson,EF