Inhibition of the growth and cysteine proteinase activity of Porphyromonas gingivalis by human salivary cystatin S and chicken cystatin.
Inhibition of the growth and cysteine proteinase activity of Porphyromonas gingivalis by human salivary cystatin S and chicken cystatin.
复制标题
人唾液胱抑素 S 和鸡胱抑素对牙龈卟啉单胞菌生长和半胱氨酸蛋白酶活性的抑制作用。
DOI：
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发表时间：
1996
影响因子：
3.7
通讯作者：
A. V. Amerongen
中科院分区：
文献类型：
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作者：
M. Blankenvoorde;Y. Henskens;W. V. Hof;E. Veerman;A. V. Amerongen
已经研究了牙龈卟啉单胞菌和几种半胱氨酸蛋白酶抑制剂种类的相互作用。在与牙龈卟啉单胞菌培养物上清液孵育后，半胱氨酸蛋白酶抑制剂S、半胱氨酸蛋白酶抑制剂C和鸡半胱氨酸蛋白酶抑制剂从14 kDa蛋白质截短为约13 kDa的多肽。截短的胱抑素S多肽的氨基酸序列分析显示，胱抑素S在Arg-8后被切割。所有三种类型的截短半胱氨酸蛋白酶抑制剂都完全保留了对木瓜蛋白酶的抑制活性。半胱氨酸蛋白酶抑制剂S和鸡半胱氨酸蛋白酶抑制剂部分抑制牙龈卟啉单胞菌培养上清的蛋白水解活性。此外，半胱氨酸蛋白酶抑制剂S和鸡半胱氨酸蛋白酶抑制剂在约1 μ M下将培养物中牙龈卟啉单胞菌的生长抑制至50%。
The mutual effects of P. gingivalis and several cystatin species has been investigated. After incubation with P. gingivalis culture supernatant, cystatin S, cystatin C and chicken cystatin were truncated from a 14 kDa protein into a polypeptide of approximately 13 kDa. Amino acid sequence analysis of the truncated cystatin S polypeptide revealed that cystatin S was cleaved after Arg-8. All three types of truncated cystatins fully retained their inhibitory activity toward papain. Cystatin S and chicken cystatin partially inhibited proteolytic activity in the culture supernatant of P. gingivalis. Furthermore, cystatin S and chicken cystatin inhibited the growth of P. gingivalis in culture to 50% at approximately 1 microM.