CANINE PARVOVIRUS SEROLOGY - A COLLABORATIVE ASSAY
CANINE PARVOVIRUS SEROLOGY - A COLLABORATIVE ASSAY
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DOI：
10.1136/vr.120.12.270
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发表时间：
1987-03-21
影响因子：
2.2
通讯作者：
THORNTON, DH
中科院分区：
文献类型：
--
作者：
LUFF, PR;WOOD, GW;THORNTON, DH
向15个实验室提供了编码的犬血清样品，用于检测是否存在犬细小病毒抗体。这些血清之一已被指定为潜在的英国标准犬细小病毒抗血清。这些实验室大多数要么提供犬细小病毒血清学服务，要么代表制药公司为联合王国市场生产犬细小病毒疫苗。未试图影响所用的试验方法。13个实验室使用了血凝抑制试验，3个实验室使用了酶联免疫吸附试验，2个实验室进行了血清中和试验。三个实验室使用了两种不同的技术。只有根据血凝抑制试验的结果才能进行充分的分析。使用标准血清可部分控制实验室间结果的变异性。大部分残差变异性与特定实验室有关。来自疫苗生产商的结果往往比来自诊断实验室的结果变化更小。
Fifteen laboratories were supplied with coded samples of canine sera for testing for the presence of antibodies against canine parvoviruses. One of these sera had been designated as a potential British standard canine parvovirus antiserum. Most of these laboratories were either providing a canine parvovirus serology service, or represented pharmaceutical companies which manufacture canine parvovirus vaccines for the United Kingdom market. No attempt was made to influence the test methods used. Thirteen of the laboratories used a haemagglutination inhibition test, three an enzyme-linked immunosorbent assay (ELISA), and two performed serum neutralisation tests. Three laboratories used two different techniques. Adequate analysis was possible only with the results of the haemagglutination inhibition tests. The variability of the results between laboratories could be partly controlled by the use of the standard serum. Much of the residual variability was associated with particular laboratories. The results from the vaccine manufacturers tended to be less variable than those from the diagnostic laboratories.