PREPARATION OF FATTY ACID METHYL ESTERS AND DIMETHYLACETALS FROM LIPIDS WITH BORON FLUORIDE--METHANOL.
PREPARATION OF FATTY ACID METHYL ESTERS AND DIMETHYLACETALS FROM LIPIDS WITH BORON FLUORIDE--METHANOL.
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DOI：
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发表时间：
1964-10
影响因子：
6.5
通讯作者：
W. R. Morrison;L. Smith
中科院分区：
文献类型：
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作者：
W. R. Morrison;L. Smith
通过用氟化硼-甲醇（每升甲醇140 g BFI）处理脂质，制备适用于气相色谱分析的脂肪酸甲酯和二甲基缩醛。该试剂稳定且易于操作。研究了甘油三酯、甘油二酯、甘油单酯、游离脂肪酸、甾醇酯、磷脂酰乙醇胺、磷脂酰丝氨酸、磷脂酰胆碱、单磷脂酰肌醇、单半乳糖甘油酯、磷脂酰胆碱（磷脂酰胆碱）、双半乳糖甘油酯和鞘磷脂的反应条件。通过薄层色谱法容易地纯化甲酯和二甲基缩醛，并且产率是定量的。几乎没有不良的副反应，它们不影响该方法的有效性。该方法简便、快速、适用于脂类样品。
Fatty acid methyl esters and dimethylacetals suitable for gas chromatographic analysis were prepared by treatment of lipids with boron fluoride-methanol (140 g BFI per liter of methanol). This reagent is stable and easy to handle. Reaction conditions were investigated for triglycerides, diglycerides, monoglycerides, free fatty acids, sterol esters, phosphatidyl ethanolamines, phosphatidyl serines, phosphatidyl 'cholines, monophosphoinositides, monogalactosyl glycerides, phosphatidal cholines (choline plasmalogens), digalactosyl glycerides, and sphingomyelins. The methyl esters and dimethylacetals were readily purified by thin-layer chromatography, and yields were quantitative. There were few undesirable side reactions, and they did not affect the validity of the method. The procedure developed is simple, rapid, and generally applicable t o lipids.