Quantitative DNA slot blot analysis: inhibition of DNA binding to membranes by magnesium ions
Quantitative DNA slot blot analysis: inhibition of DNA binding to membranes by magnesium ions
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DOI：
10.1093/nar/25.16.3375
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发表时间：
1997-08-15
影响因子：
14.9
通讯作者：
Srivastava, A
中科院分区：
文献类型：
--
作者：
Kube, DM;Srivastava, A
野生型和重组腺相关病毒滴度通过DNA槽印迹分析常规测定。发现病毒DNA与尼龙膜的结合被镁离子抑制，镁离子是在凹槽印迹分析之前进行的DNase I消化的关键成分。Mg2+离子也干扰了质粒DNA在尼龙和硝化纤维膜上的吸附。这些观察结果对DNA分子在固体载体上保留的鲜为人知的机制产生了实际的见解。
Titers of wild-type and recombinant adeno-associated viruses are routinely determined by DNA slot blot analysis. The binding of viral DNA to nylon membranes was found to be inhibited by magnesium ions, which are critical components of the DNase I digestion carried out prior to slot blot analysis. Mg2+ ions also interfered with the adsorption of plasmid DNA to nylon and nitrocellulose membranes. These observations yield practical insights into the poorly understood mechanisms by which DNA molecules are retained on solid supports.