Microbial community composition in iron deposits and manganese crusts formed in riverine environments around the Aso area in Japan
Microbial community composition in iron deposits and manganese crusts formed in riverine environments around the Aso area in Japan
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DOI：
10.1016/j.resmic.2020.09.003
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发表时间：
2020-10-01
影响因子：
2.6
通讯作者：
Oyama, Takahiro
中科院分区：
文献类型：
--
作者：
Hirano, Shin-ichi;Ito, Yuki;Oyama, Takahiro
流经日本西南部阿索破火山口的黑川受到含有高浓度溶解金属（Fe 2+和Mn 2+）的地下水的影响，上游有橙子沉积物，中游地区有几公里长的人工水道隧道壁上有厚的黑色结壳。X射线荧光分析表明，橙子矿床含有高达34.4%的铁和黑色结壳分别高达25.4%和10.6%的锰和铁。虽然自然发生的锰氧化物和结壳被认为是生物在pH值中性的淡水环境中，锰结壳中的整个微生物群落及其在这些环境中对锰结壳形成的贡献仍然不清楚。高通量分子测序的细菌和真核生物，包括真菌显示黑色锰结壳有一个高丰度的鞘氨醇单胞菌属，丝微菌属，芽孢杆菌属，假单胞菌属，被孢霉属，以前报道的属，包括几个物种与锰氧化活性的锰结壳或结核的其他海洋和淡水环境。此外，从黑锰结壳中分离到一株具有锰氧化活性的细菌和一株具有锰氧化活性的真菌。这些结果表明，包括假单胞菌和真菌在内的多种锰氧化细菌参与了黑川河贫营养环境中暗水道隧道壁上锰结壳的形成。(C)2020年巴斯德研究所。由Elsevier Masson SAS出版。All rights reserved.
The Kurokawa river flowing through the Aso Caldera in Southwest Japan is influenced by groundwater containing high concentration of dissolved metals (Fe2+ and Mn2+) and is associated with orange deposits in the upstream and thick black crusts on the wall of artificial waterway tunnel with several km lengths in the midstream regions. X-ray fluorescence analysis revealed that the orange deposits contained up to 34.4% Fe and black crusts comprised up to 25.4% and 10.6% Mn and Fe, respectively. Although naturally occurring Mn-oxides and crusts are considered biogenic in pH-neutral freshwater environments, the whole microbial community in Mn crust and their contribution to Mn crust formation in these environments remain unclear. High-throughput molecular sequencing for bacteria and eukaryotes including fungi revealed black Mn crusts had a high abundance of Sphingomonas, Hyphomicrobium, Bacillus, Pseudomonas, and Mortierella, previously reported genera including several species with Mn-oxidizing activity in Mn crusts or nodules of other marine and freshwater environments. In addition, one bacterial isolate and one fungal isolate with Mn-oxidizing activity were obtained from black Mn crust. These results suggest that multiple Mn-oxidizing bacteria including Pseudomonas and fungi are involved in Mn crust formation on the wall of dark waterway tunnel in the oligotrophic environment of the Kurokawa river. (C) 2020 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.