Propofol prevents lipid peroxidation following transient forebrain ischemia in gerbils
Propofol prevents lipid peroxidation following transient forebrain ischemia in gerbils
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DOI：
10.1007/bf03024877
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发表时间：
2000-10
期刊：
影响因子：
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通讯作者：
S. Yamaguchi;S. Hamaguchi;M. Mishio;Y. Okuda;T. Kitajima
中科院分区：
文献类型：
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作者：
S. Yamaguchi;S. Hamaguchi;M. Mishio;Y. Okuda;T. Kitajima
目的：探讨异丙酚对沙鼠海马CAI亚区短暂性前脑缺血致延迟性神经元死亡的脂质过氧化作用。方法：将40只沙鼠随机分为5组：1组为对照组，假手术后给予生理盐水处理；II组，PSS治疗缺血/再灌注；III组，50 mg·kg−1异丙酚处理缺血/再灌注；IV组，100 mg·kg−1异丙酚处理缺血/再灌注；V组，150 mg·kg−1异丙酚处理缺血/再灌注。异丙酚或PSS麻醉后，在N2O/O2/氟烷麻醉下阻断双侧颈总动脉4分钟，诱导短暂性前脑缺血。5天后，大脑被移除，每个前脑被切成两个，包括海马体。利用丙二醛（MDA）的产生来测定脂质过氧化，并检查海马CAI亚场的组织病理学变化。结果：II组锥体细胞萎缩、收缩，径向条纹区出现空泡化和结构破坏。在其他四组中，没有观察到这些变化。锥体细胞退行性比：I组：4.9±2.3，II组：94.1±4.5 (P<0.01)， III组：12.5±5.7，IV组：11.0±4.6，V组：9.6±4.9%。丙二醛（MDA）产生量：ⅰ组：83±22，ⅱ组：198±25 (P<0.01)，ⅲ组：153±39，ⅳ组：113±34，ⅴ组：106±27 nmol·g−1。结论：异丙酚可通过预防沙鼠海马CAI亚区短暂性前脑缺血引起的脂质过氧化而减轻延迟性神经元死亡。
Purpose:To ascertain whether propofol prevents lipid peroxidation on delayed neuronal death induced by transient forebrain ischemia in the hippocampal CAI subfield in gerbils.Methods:Forty gerbils were randomly assigned to five groups: Group I, control, sham operation treated with physiological saline solution (PSS); Group II, ischemia/reperfusion treated with PSS; Group III, ischemia/reperfusion treated with 50 mg·kg−1propofol; Group IV, ischemia/reperfusion treated with 100 mg·kg−1propofol; Group V, ischemia/reperfusion treated with 150 mg·kg−1propofol. Transient forebrain ischemia was induced by occluding the bilateral common carotid arteries for four minutes under N2O/O2/halothane anesthesia after propofol or PSS. Five days later, the cerebrum was removed and each forebrain was cut into two including the hippocampus. Lipid peroxidation was determined using the production of malondialdehyde (MDA), and histopathological changes in the hippocampal CAI subfield were examined.Results:In group II, the pyramidal cells were atrophic and pycnotic, vacuolation and structural disruption of the radial striated zone was observed. In the other four groups, these changes were not observed. Degenerative ratios of pyramidal cells were: Group I: 4.9±2.3, Group II: 94.1±4.5 (P<0.01), Group III: 12.5±5.7, Group IV: 11.0±4.6, Group V: 9.6±4.9%. Production of MDA was: Group I: 83±22, Group II: 198±25 (P<0.01), Group III: 153±39, Group IV: 113±34, Group V: 106±27 nmol·g−1wet tissue.Conclusion:Propofol attenuated delayed neuronal death by preventing lipid peroxidation induced by transient forebrain ischemia in the hippocampal CAI subfield in gerbils.