Molecular evidence of Ebola Reston virus infection in Philippine bats.
Molecular evidence of Ebola Reston virus infection in Philippine bats.
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DOI：
10.1186/s12985-015-0331-3
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发表时间：
2015-07-17
期刊：
影响因子：
4.8
通讯作者：
Newman SH
中科院分区：
文献类型：
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作者：
Jayme SI;Field HE;de Jong C;Olival KJ;Marsh G;Tagtag AM;Hughes T;Bucad AC;Barr J;Azul RR;Retes LM;Foord A;Yu M;Cruz MS;Santos IJ;Lim TM;Benigno CC;Epstein JH;Wang LF;Daszak P;Newman SH
2008-09年，在菲律宾的家猪和养猪工人中发现了雷斯顿埃博拉病毒(RESTV)感染的证据。由于蝙蝠物种已被证明是其他地方丝状病毒的隐蔽宿主，菲律宾政府与联合国粮食及农业组织联合成立了一个多学科、多机构的小组，以调查菲律宾蝙蝠可能是RESTV的宿主。该小组在2010年期间利用血清学和分子分析对多个地点的蝙蝠种群进行了监测。共有21个物种的464只蝙蝠进行了采样。我们在多种蝙蝠中发现了RESTV感染的分子和血清学证据。用定量聚合酶链式反应(QPCR)检测了施氏小翅目小翅目鱼口咽拭子中的RNA，其中3个样本在常规半套式聚合酶链式反应中扩增出与菲律宾猪分离株序列相差1个核苷酸的产物。未经证实的qPCR检测可能表明在其他几种蝙蝠中存在RESTV核酸(澳洲蝙蝠、短毛蝙蝠和短毛蝙蝠)。褶皱)。我们还用免疫印迹和ELISA法检测了三只蝙蝠(Acerodon Jubatus)的抗RESTV抗体。这些发现表明，埃博拉病毒在菲律宾蝙蝠中的感染在分类学上是普遍存在的，但明显的低流行率和低病毒载量需要扩大监测，以详细阐述研究结果，并更广泛地确定埃博拉病毒在该地区蝙蝠中的分类和地理分布。
In 2008–09, evidence of Reston ebolavirus (RESTV) infection was found in domestic pigs and pig workers in the Philippines. With species of bats having been shown to be the cryptic reservoir of filoviruses elsewhere, the Philippine government, in conjunction with the Food and Agriculture Organization of the United Nations, assembled a multi-disciplinary and multi-institutional team to investigate Philippine bats as the possible reservoir of RESTV. The team undertook surveillance of bat populations at multiple locations during 2010 using both serology and molecular assays. A total of 464 bats from 21 species were sampled. We found both molecular and serologic evidence of RESTV infection in multiple bat species. RNA was detected with quantitative PCR (qPCR) in oropharyngeal swabs taken from Miniopterus schreibersii, with three samples yielding a product on conventional hemi-nested PCR whose sequences differed from a Philippine pig isolate by a single nucleotide. Uncorroborated qPCR detections may indicate RESTV nucleic acid in several additional bat species (M. australis, C. brachyotis and Ch. plicata). We also detected anti-RESTV antibodies in three bats (Acerodon jubatus) using both Western blot and ELISA. The findings suggest that ebolavirus infection is taxonomically widespread in Philippine bats, but the evident low prevalence and low viral load warrants expanded surveillance to elaborate the findings, and more broadly, to determine the taxonomic and geographic occurrence of ebolaviruses in bats in the region.