Synthesis of fluorapatite-hydroxyapatite nanoparticles and toxicity investigations
Synthesis of fluorapatite-hydroxyapatite nanoparticles and toxicity investigations
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DOI：
10.2147/ijn.s15461
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发表时间：
2011-01-01
影响因子：
8
通讯作者：
Biazar, Esmaeil
中科院分区：
文献类型：
--
作者：
Montazeri, N.;Jahandideh, R.;Biazar, Esmaeil
本研究采用溶胶凝胶法制备了含氟磷灰石(FA；Ca-10(PO4)(6)F-2)和羟基磷灰石(HA；Ca-10(PO4)(6)(OH)(2))两种不同晶相的纳米磷酸钙。分别使用磷酸乙酯、水合硝酸钙和氟化铵作为磷、钙和氟的前驱体，钙磷比为1：72。对经80℃干燥、550℃热处理后的溶胶-凝胶法制备的粉末进行了表征，通过扫描电子显微镜、X射线衍射仪、傅立叶变换红外光谱和Zetasizer实验对粉末的结晶度、颗粒和微晶尺寸、粉末形貌、化学结构和物相分析进行了研究。X-射线衍射仪和傅里叶变换红外光谱分析表明，样品中存在羟基磷灰石和氟磷灰石两种相。用Scherrer方程计算得到的微晶尺寸约为20 nm，羟基磷灰石相的结晶度约为70%。透射电子显微镜、扫描电子显微镜图像和Zetasizer实验表明，其平均尺寸为100 nm。以小鼠成纤维细胞为研究对象，考察了粉剂的体外行为。实验结果表明，该粉末具有良好的生物相容性，不会引起毒性反应。这些化合物可以应用于硬组织工程。
In this study, calcium phosphate nanoparticles with two phases, fluorapatite (FA; Ca-10(PO4)(6)F-2) and hydroxyapatite (HA; Ca-10(PO4)(6)(OH)(2)), were prepared using the sol-gel method. Ethyl phosphate, hydrated calcium nitrate, and ammonium fluoride were used, respectively, as P, Ca, and F precursors with a Ca: P ratio of 1:72. Powders obtained from the sol-gel process were studied after they were dried at 80 degrees C and heat treated at 550 degrees C. The degree of crystallinity, particle and crystallite size, powder morphology, chemical structure, and phase analysis were investigated by scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and Zetasizer experiments. The results of XRD analysis and FTIR showed the presence of hydroxyapatite and fluorapatite phases. The sizes of the crystallites estimated from XRD patterns using the Scherrer equation and the crystallinity of the hydroxyapatite phase were about 20 nm and 70%, respectively. Transmission electron microscope and SEM images and Zetasizer experiments showed an average size of 100 nm. The in vitro behavior of powder was investigated with mouse fibroblast cells. The results of these experiments indicated that the powders were biocompatibile and would not cause toxic reactions. These compounds could be applied for hard-tissue engineering.