IMMUNOELECTRON MICROSCOPIC LOCALIZATION OF HLA-DR ANTIGEN IN CONTROL SMALL-INTESTINE AND COLON AND IN INFLAMMATORY BOWEL-DISEASE
IMMUNOELECTRON MICROSCOPIC LOCALIZATION OF HLA-DR ANTIGEN IN CONTROL SMALL-INTESTINE AND COLON AND IN INFLAMMATORY BOWEL-DISEASE
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DOI：
10.1007/bf01299810
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发表时间：
1986-12-01
影响因子：
3.1
通讯作者：
DOBBINS, WO
中科院分区：
文献类型：
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作者：
HIRATA, I;AUSTIN, LL;DOBBINS, WO
我们使用免疫电子显微镜方法阐明了 I2 (HLA-DR) 抗原在对照和炎症性肠病标本中的分布。对照小肠上皮和炎症性肠病上皮表达12抗原，而对照结肠上皮则不表达。肠上皮细胞的 I2 表达在侧表面和基底表面比在微绒毛表面更频繁。对照回肠中的三个 M 细胞中有两个表达 I2 抗原。 I2 阳性固有层淋巴细胞在炎症性肠病中显着增加，而 I2 阳性固有层淋巴细胞在对照标本中几乎不存在。在对照和炎症性肠病标本中，肠固有层中的 I2 阳性单核细胞主要是巨噬细胞和单核细胞。在对照或疾病标本中未检测到类似树突状细胞的 I2 阳性单核细胞。此外，对照样本和疾病样本中 I2 阳性或阴性巨噬细胞和单核细胞没有显着的形态学差异。雪旺细胞上 I2 抗原的表达在疾病标本中比在对照标本中更频繁地检测到。对照和疾病标本的毛细血管内皮均表达 I2 抗原。我们证明 I2 表达存在于肠道和结肠的免疫和非免疫细胞的表面膜上，并且表明这种表达在炎症性肠病中比在对照肠道和结肠中更为突出。需要进一步的研究来确定这一发现在抗原呈递方面是否有意义以及这种明显的“免疫激活”是否与炎症性肠病的发病机制有关。
We have elucidated the distribution of I2 (HLA-DR) antigen in control and inflammatory bowel disease specimens, using immunoelectron microscopic methods. Control small intestinal epithelium and inflammatory bowel disease epithelium expressed 12 antigen, while control colonic epithelium did not. I2 expression by enterocytes was more frequent on the lateral and basal surface than on the microvillus surface. Two of three M cells in control ileum expressed I2 antigen. I2-positive lamina propria lymphocytes were significantly increased in inflammatory bowel disease, while I2-positive lamina propria lymphocytes were virtually absent in control specimens. I2-positive mononuclear cells in the intestinal lamina propria were largely macrophages and monocytes in both control and inflammatory bowel disease specimens. I2-positive mononuclear cells resembling dendritic cells were not detected in control or disease specimens. Furthermore, there were no significant morphological differences in I2-positive or -negative macrophages and monocytes in control and disease specimens. The expression of I2 antigen on Schwann cells was detected more frequently in disease specimens than in control specimens. Capillary endothelia of both control and disease specimens expressed I2 antigen. We demonstrate that I2 expression is present on surface membranes of both immune and nonimmune cells of the intestine and colon and show that this expression is more prominent in inflammatory bowel disease than in control intestine and colon. Further studies are required to determine whether this finding is meaningful in terms of antigen presentation and whether this apparent "immune activation" is involved in the pathogenesis of inflammatory bowel disease.