Detection of seed dormancy QTL in multiple mapping populations derived from crosses involving novel barley germplasm
Detection of seed dormancy QTL in multiple mapping populations derived from crosses involving novel barley germplasm
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DOI：
10.1007/s00122-007-0620-3
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发表时间：
2007-10-01
影响因子：
5.4
通讯作者：
Takeda, Kazuyoshi
中科院分区：
文献类型：
--
作者：
Hori, Kiyosumi;Sato, Kazuhiro;Takeda, Kazuyoshi
大麦种子休眠性是控制发芽和穗发芽的重要性状。基于EST的连锁图谱构建7重组自交系（RI）和一个双单倍体（DH）的杂交群体，包括11个栽培和野生大麦品种显示广泛的种子休眠水平。在2003年和2005年，从收获后成熟期5周和10周的发芽率估计每个RI和DH系的种子休眠。利用复合区间作图法对RI和DH群体进行了控制种子休眠性的数量性状基因座（QTL）定位。在8个大麦群体中，除2H外，共检测到38个QTL，分布在11个区域。在本研究中检测到的几个QTL区域在以前的QTL研究中报道的相似位置。在不同休眠深度和休眠期的RI和DH群体中均检测到位于5H染色体长臂着丝粒区的QTL。该QTL的作用基因可能在杂交组合间存在较大的等位变异，也可能是位于同一区域的多个基因。本研究中发现的大麦种质中的不同位点及其对休眠的不同影响使我们能够在大麦育种计划中控制种子休眠的实际水平。
Seed dormancy is one of the most important traits in germination process to control malting and pre-harvest sprouting in barley (Hordeum vulgare L.). EST based linkage maps were constructed on seven recombinant inbred (RI) and one doubled haploid (DH) populations derived from crosses including eleven cultivated and one wild barley strains showing the wide range of seed dormancy levels. Seed dormancy of each RI and DH line was estimated from the germination percentage at 5 and 10 weeks post-harvest after-ripening periods in 2003 and 2005. Quantitative trait loci (QTLs) controlling seed dormancy were detected by the composite interval mapping procedure on the RI and DH populations. A total of 38 QTLs clustered around 11 regions were identified on the barley chromosomes except 2H among the eight populations. Several QTL regions detected in the present study were reported on similar positions in the previous QTL studies. The QTL on at the centromeric region of long arm of chromosome 5H was identified in all the RI and DH populations with the different degrees of dormancy depth and period. The responsible gene of the QTL might possess a large allelic variation among the cross combinations, or can be multiple genes located on the same region. The various loci and their different effects in dormancy found in the barley germplasm in the present study enable us to control the practical level of seed dormancy in barley breeding programs.