The centromeric nucleosome-like CENP-T-W-S-X complex induces positive supercoils into DNA.
The centromeric nucleosome-like CENP-T-W-S-X complex induces positive supercoils into DNA.
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DOI：
10.1093/nar/gkt1124
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发表时间：
2014-02
影响因子：
14.9
通讯作者：
Fukagawa T
中科院分区：
文献类型：
--
作者：
Takeuchi K;Nishino T;Mayanagi K;Horikoshi N;Osakabe A;Tachiwana H;Hori T;Kurumizaka H;Fukagawa T
着丝粒是一个特殊的基因组区域，其上形成的动粒附着在纺锤体微管上，以实现染色体的可靠分离。为了将该染色体区域与其他基因组基因座区分开，着丝粒含有特定的染色质结构，包括含有组蛋白H3变体CENP-A的特化核小体。除了CENP-A核小体外，我们还发现着丝粒含有由组蛋白折叠CENP-T-W-S-X复合物组成的核小体样结构。然而，CENP-T-W-S-X复合物如何与着丝粒染色质结合尚不清楚。在这里，我们证明了CENP-T-W-S-X复合物优先结合到100 bp的接头DNA，而不是核小体结合的DNA。此外，我们发现CENP-T-W-S-X复合物主要以（CENP-T-W-S-X）2结构与DNA结合。有趣的是，与负超螺旋DNA的典型核小体相反，CENP-T-W-S-X复合物诱导正DNA超螺旋。我们发现，CENP-T或CENP-W中的DNA结合区域，而不是CENP-S或CENP-X，是这种正超螺旋活性和CENP-T-W-S-X复合物的动粒靶向所必需的。总之，我们的工作揭示了CENP-T-W-S-X复合物的结构特征和定位于着丝粒的性质。
The centromere is a specific genomic region upon which the kinetochore is formed to attach to spindle microtubules for faithful chromosome segregation. To distinguish this chromosomal region from other genomic loci, the centromere contains a specific chromatin structure including specialized nucleosomes containing the histone H3 variant CENP–A. In addition to CENP–A nucleosomes, we have found that centromeres contain a nucleosome-like structure comprised of the histone-fold CENP–T–W–S–X complex. However, it is unclear how the CENP–T–W–S–X complex associates with centromere chromatin. Here, we demonstrate that the CENP–T–W–S–X complex binds preferentially to ∼100 bp of linker DNA rather than nucleosome-bound DNA. In addition, we find that the CENP–T–W–S–X complex primarily binds to DNA as a (CENP–T–W–S–X)2 structure. Interestingly, in contrast to canonical nucleosomes that negatively supercoil DNA, the CENP–T–W–S–X complex induces positive DNA supercoils. We found that the DNA-binding regions in CENP–T or CENP–W, but not CENP–S or CENP–X, are required for this positive supercoiling activity and the kinetochore targeting of the CENP–T–W–S–X complex. In summary, our work reveals the structural features and properties of the CENP–T–W–S–X complex for its localization to centromeres.
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影响因子：
11.8
作者：
Shang, Wei-Hao;Hori, Tetsuya;Martins, Nuno M. C.;Toyoda, Atsushi;Misu, Sadahiko;Monma, Norikazu;Hiratani, Ichiro;Maeshima, Kazuhiro;Ikeo, Kazuho;Fujiyama, Asao;Kimura, Hiroshi;Earnshaw, William C.;Fukagawa, Tatsuo
通讯作者：
Fukagawa, Tatsuo
影响因子：
1.6
作者：
Henikoff S;Furuyama T
通讯作者：
Furuyama T
影响因子：
9.8
作者：
Dalal Y;Wang H;Lindsay S;Henikoff S
通讯作者：
Henikoff S
影响因子：
64.5
作者：
Nishino T;Takeuchi K;Gascoigne KE;Suzuki A;Hori T;Oyama T;Morikawa K;Cheeseman IM;Fukagawa T
通讯作者：
Fukagawa T
影响因子：
64.5
作者：
Hori, Tetsuya;Amano, Miho;Fukagawa, Tatsuo
通讯作者：
Fukagawa, Tatsuo