Effect of quercetin on chondrocyte phenotype and extracellular matrix expression
Effect of quercetin on chondrocyte phenotype and extracellular matrix expression
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槲皮素对软骨细胞表型和细胞外基质表达的影响
DOI：
10.1016/s1875-5364(20)60036-x
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发表时间：
2020-12-01
影响因子：
4.6
通讯作者：
Xu Yuan-Jin
中科院分区：
文献类型：
--
作者：
Gui Zhi-Peng;Hu Yue;Xu Yuan-Jin
由于软骨组织的修复能力较差，再生医学在修复大面积关节软骨缺损方面仍面临巨大挑战。槲皮素作为一种中药被广泛应用于组织再生，包括肝脏，骨骼和皮肤组织。然而，其对软骨再生的影响和内部机制的证据是有限的。本研究通过CCK 8测定、PCR测定、软骨基质染色测定、免疫荧光测定和蛋白质印迹法系统评价槲皮素对软骨细胞功能的影响。结果表明，槲皮素通过剂量依赖性激活ERK、P38和AKT信号通路，显著上调软骨形成相关基因的表达，促进糖胺聚糖（glycosaminoglycan，GAG）的分泌。此外，体内实验表明，槲皮素负载的丝蛋白支架显着刺激新的软骨样组织的形成，在大鼠股骨软骨缺损具有较高的组织学评分。这些数据表明，槲皮素可以有效地刺激软骨在体外和体内的形成，展示了潜在的应用，槲皮素在软骨缺损的再生。
Due to the poor repair ability of cartilage tissue, regenerative medicine still faces great challenges in the repair of large articular cartilage defects. Quercetin is widely applied as a traditional Chinese medicine in tissue regeneration including liver, bone and skin tissues. However, the evidence for its effects and internal mechanisms for cartilage regeneration are limited. In the present study, the effects of quercetin on chondrocyte function were systematically evaluated by CCK8 assay, PCR assay, cartilaginous matrix staining assays, immunofluorescence assay, and western blotting. The results showed that quercetin significantly up-regulated the expression of chondrogenesis genes and stimulated the secretion of GAG (glycosaminoglycan) through activating the ERK, P38 and AKT signalling pathways in a dose-dependent manner. Furthermore, in vivo experiments revealed that quercetin-loaded silk protein scaffolds dramatically stimulated the formation of new cartilage-like tissue with higher histological scores in rat femoral cartilage defects. These data suggest that quercetin can effectively stimulate chondrogenesis in vitro and in vivo, demonstrating the potential application of quercetin in the regeneration of cartilage defects.