HLA DQ alleles and interleukin-10 polymorphism associated with Chlamydia trachomatis-related tubal factor infertility:: a case-control study
HLA DQ alleles and interleukin-10 polymorphism associated with Chlamydia trachomatis-related tubal factor infertility:: a case-control study
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DOI：
10.1093/humrep/17.8.2073
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发表时间：
2002-08-01
影响因子：
6.1
通讯作者：
Paavonen, J
中科院分区：
文献类型：
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作者：
Kinnunen, AH;Surcel, HM;Paavonen, J
背景：研究了沙眼衣原体输卵管因素不孕症（TFI）与宿主免疫调节基因的关系。方法：通过淋巴细胞增殖试验测定细胞介导的针对沙眼衣原体和衣原体热休克蛋白（CHSP60）的免疫反应。通过 PCR 分析了 52 名 TFI 病例和 61 名对照者的 HLA-DQ 等位基因和白细胞介素 10 (IL-10) 启动子多态性 (-1082 A/G)。结果：TFI 组和对照组之间的 HLA-DQB1 或 DQA1 等位基因没有显着差异。然而，DQA1*0102 和 DQB1*0602 等位基因以及 IL-10 -1082AA 基因型在 TFI 患者中的出现频率显着高于对照组（分别为 0.18 和 0.02；P = 0.005）。对 CHSP60 呈阳性淋巴细胞增殖反应的 23 名患者中，有 5 名 (22%) 的 IL-10 -1082AA 以及 HLA-DQA1*0102 和 HLA-DQB1*0602 等位基因也呈阳性。结论：我们的结果揭示了衣原体 TFI 患者的细胞免疫反应与 CHSP60、HLA II 类等位基因和 IL-10 启动子基因型之间的关联。
BACKGROUND: The relationship between Chlamydia trachomatis tubal factor infertility (TFI) and the host's immunoregulatory genes was studied. METHODS: Cell-mediated immune responses to C. trachomatis and chlamydial heat shock protein (CHSP60) were determined by lymphocyte proliferation assay. HLA-DQ alleles and interleukin-10 (IL-10) promoter polymorphism (-1082 A/G) were analysed in 52 TFI cases and in 61 controls by PCR. RESULTS: HLA-DQB1 or DQA1 alleles did not significantly differ between the TFI group and the control group. However, DQA1*0102 and DQB1*0602 alleles together with IL-10 -1082AA genotype were found significantly more frequently in the TFI patients than in the controls (0.18 and 0.02 respectively; P = 0.005). Five (22%) of the 23 patients who had a positive lymphocyte proliferative response to CHSP60 were positive also for IL-10 -1082AA and for the HLA-DQA1*0102 and HLA-DQB1*0602 alleles. CONCLUSIONS: Our results reveal an association of a cellular immune response to CHSP60, HLA class II alleles and IL-10 promoter genotypes in patients with chlamydial TFI.