Uptake characteristics of mannosylated and fucosylated bovine serum albumin in primary cultured rat sinusoidal endothelial cells and Kupffer cells
Uptake characteristics of mannosylated and fucosylated bovine serum albumin in primary cultured rat sinusoidal endothelial cells and Kupffer cells
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DOI：
10.1016/j.ijpharm.2004.08.021
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发表时间：
2004-12-09
影响因子：
5.8
通讯作者：
Hashida, M
中科院分区：
文献类型：
--
作者：
Higuchi, Y;Nishikawa, M;Hashida, M
本研究的目的是描述原代培养的肝窦内皮细胞和枯否细胞对甘露糖基化和岩藻糖基化蛋白的摄取特性。在培养的窦状隙内皮细胞，摄取的甘露糖基化和岩藻糖基化牛血清白蛋白（BSA）的显着抑制过量的甘露糖基化和岩藻糖基化BSA，但不是由半乳糖基化BSA，这表明这两种糖基化蛋白可能主要通过甘露糖受体。在培养的Kupffer细胞中，岩藻糖基化BSA的摄取被过量的半乳糖基化BSA以及甘露糖基化和岩藻糖基化BSA显著抑制，尽管甘露糖基化BSA仅被甘露糖基化和岩藻糖基化BSA抑制。这表明枯否细胞对岩藻糖基化BSA的摄取可能由枯否细胞凝集素（岩藻糖受体）和甘露糖受体介导。另一方面，在体内的岩藻糖基化BSA的肝摄取抑制到更大程度上由GdCl 3预处理比甘露糖基化BSA。基于体外和体内实验，可以得出结论，岩藻糖基化BSA具有更高的枯否细胞选择性，因为它表现出比甘露糖基化BSA更低的窦状内皮细胞摄取。(C)2004 Elsevier B.V.保留所有权利。
The purpose of this study is to delineate uptake characteristics of mannosylated and fucosylated proteins in primary cultured sinusoidal endothelial cells and Kupffer cells. In cultured sinusoidal endothelial cells, uptake of mannosylated and fucosylated bovine serum albumin (BSA) was significantly inhibited by excess mannosylated and fucosylated BSAs but not by galactosylated BSA, suggesting that both glycosylated proteins might be primarily taken up via mannose receptors. In cultured Kupffer cells, uptake of fucosylated BSA was significantly inhibited by excess galactosylated BSA as well as mannosylated and fucosylated BSAs, although that of mannosylated BSA was inhibited only by mannosylated and fucosylated BSAs. This suggests that uptake of fucosylated BSA by Kupffer cells might be mediated by both Kupffer cell lectin (fucose receptor) and mannose receptor. On the other hand, in vivo hepatic uptake of fucosylated BSA was inhibited to a greater extent by GdCl3 pretreatment than that of mannosylated BSA. Based on in vitro and in vivo experiments, it was concluded that fucosylated BSA is more Kupffer cell-selective because it exhibited a lower sinusoidal endothelial cell uptake than mannosylated BSA. (C) 2004 Elsevier B.V. All rights reserved.