Rose Phytoene Desaturase Gene Silencing by Apple Latent Spherical Virus Vectors
Rose Phytoene Desaturase Gene Silencing by Apple Latent Spherical Virus Vectors
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DOI：
10.21273/hortsci.47.9.1278
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发表时间：
2012-09
期刊：
影响因子：
1.9
通讯作者：
Hiroaki Ito;M. Ochiai;H. Kato;K. Shiratake;D. Takemoto;S. Otagaki;S. Matsumoto
中科院分区：
文献类型：
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作者：
Hiroaki Ito;M. Ochiai;H. Kato;K. Shiratake;D. Takemoto;S. Otagaki;S. Matsumoto
利用苹果潜伏球形病毒（ALSV）载体成功地建立了月季病毒诱导的基因沉默（VIGS）。在玫瑰上的ALSV感染没有引起任何症状，就像在其他植物物种上观察到的那样，并且生长健康。我们克隆并测序了野生玫瑰八氢番茄红素去饱和酶（PDS）基因，并利用其片段沉默了玫瑰内部的PDS基因。在一次枝上部叶和一次枝上观察到了PDS抑制的高度一致的光漂白表型等沉默表型。皱纹超过5个月。ALSV载体可用于月季基因功能分析和分子育种。
We havesucceededin establishingavirus-inducedgene silencing(VIGS)of rose using Apple latent spherical virus (ALSV) vectors. An ALSV infection on rose did not cause any symptoms like those observed on other plant species and grew healthy. We have cloned and sequenced the phytoene desaturase (PDS) gene in wild rose, then used its fragment for silencing the rose internal PDS gene. The silencing phenotypes such as the highly uniform photo-bleached phenotype with PDS inhibitions were observed on the upper leaves of primary shoots and on a secondary shoot of R. rugosa for more than 5 months. ALSV vectors seemed useful for analyzing gene function and for the molecular breeding of rose.