Streptococcus chosunense sp. nov., Isolated from Human Postoperative Maxillary Cyst
Streptococcus chosunense sp. nov., Isolated from Human Postoperative Maxillary Cyst
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DOI：
10.1007/s00284-019-01746-0
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发表时间：
2019-10-01
影响因子：
2.6
通讯作者：
Kook, Joong-Ki
中科院分区：
文献类型：
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作者：
Lim, Yun Kyong;Park, Soon-Nang;Kook, Joong-Ki
从人类上颌囊肿术后分离到一株新的兼性厌氧、不产芽孢、不运动、革兰氏染色阳性的球菌，命名为ChDC B353（T）。该菌株的16 S核糖体RNA基因（16 S rDNA）序列与假乳链球菌ATCC BAA-960（T）（99.4%）、缓症链球菌NCTC 12261（T）（99.3%）和肺炎链球菌NCTC 7465（T）（99.2%）的序列最接近。该菌株的主要脂肪酸为C-16：0（43.2%）和C-18：1 omega 6c/C-18：1 omega 7 c（20.2%）。菌株ChDC B353（T）的基因组由1，902，053 bp组成。DNA G+C含量为40.2mol%。菌株ChDC B353（T）和S之间的平均核苷酸同一性（ANI）值。假单胞菌ATCC BAA-960（T）、S. mitis NCTC 12261（T）和S. pneumoniae NCTC 7465（T）的阳性率分别为91.9%、93.5%和91.3%。ChDC B353（T）与S.假单胞菌ATCC BAA-960（T）、S. mitis NCTC 12261（T）或S. pneumoniae NCTC 7465（T）的阳性率分别为46.6%（44.0-49.2%）、53.2%（50.5-55.9%）和46.0%（43.5-48.7%）。ANI和GGD的种鉴别阈值分别为95-96%和70%。这些结果表明菌株ChDC B353（T）（= KCOM 1699（T）= JCM 33453（T））是链球菌属的一个新种，建议将其命名为朝鲜链球菌（Streptococcus chosunense sp. nov.）。
A novel facultative anaerobic, non-spore forming, non-motile, and Gram-stain-positive coccus, designated strain ChDC B353(T), was isolated from human postoperative maxillary cyst. The 16S ribosomal RNA gene (16S rDNA) sequence of the strain was most closely related to those of Streptococcus pseudopneumoniae ATCC BAA-960(T) (99.4%), Streptococcus mitis NCTC 12261(T) (99.3%), and Streptococcus pneumoniae NCTC 7465(T) (99.2%). The major fatty acids of the strain were C-16:0 (43.2%) and C-18:1 omega 6c/C-18:1 omega 7c (20.2%). The genome of strain ChDC B353(T) was composed of 1,902,053 bps. The DNA G+C content of the strain was 40.2 mol%. Average nucleotide identity (ANI) values between strain ChDC B353(T) and S. pseudopneumoniae ATCC BAA-960(T), S. mitis NCTC 12261(T), and S. pneumoniae NCTC 7465(T) were 91.9%, 93.5%, and 91.3%, respectively. Genome-to-genome distance (GGD) values between strain ChDC B353(T) and S. pseudopneumoniae ATCC BAA-960(T), S. mitis NCTC 12261(T), or S. pneumoniae NCTC 7465(T) were 46.6% (44.0-49.2%), 53.2% (50.5-55.9%), and 46.0% (43.5-48.7%), respectively. The threshold values of ANI and GGD for species discrimination are 95-96% and 70%, respectively. These results reveal that strain ChDC B353(T) (= KCOM 1699(T) = JCM 33453(T)) is a novel species belonging to genus Streptococcus, for which a name of Streptococcus chosunense sp. nov. is proposed.