Characterization of Antimicrobial Susceptibility and Its Association with Virulence Genes Related to Adherence, Invasion, and Cytotoxicity in Campylobacter jejuni and Campylobacter coli Isolates from Animals, Meat, and Humans
Characterization of Antimicrobial Susceptibility and Its Association with Virulence Genes Related to Adherence, Invasion, and Cytotoxicity in Campylobacter jejuni and Campylobacter coli Isolates from Animals, Meat, and Humans
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DOI：
10.1089/mdr.2015.0055
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发表时间：
2016-07-01
影响因子：
2.6
通讯作者：
Vidal, Roberto
中科院分区：
文献类型：
--
作者：
Lapierre, Lisette;Gatica, Maria A.;Vidal, Roberto
本研究的目的是统计分析红霉素，庆大霉素，环丙沙星和四环素的抗菌药物敏感性/耐药性之间的关联和11个毒力基因相关的粘附，侵袭和细胞毒性的528株大肠弯曲杆菌和空肠弯曲杆菌从零售肉类和粪便样本的食品生产动物和人类患者。高比例的弯曲杆菌菌株对抗菌剂，特别是环丙沙星和四环素具有耐药性。此外，我们观察到广泛分布的毒力基因的分析菌株。C.空肠弯曲菌对抗生素的敏感性高于C.大肠杆菌菌株。与入侵能力相关的基因，如racR，ciaB和pldA，与两个物种中的抗生素敏感菌株相关。基因cdtA和dnaJ，一个citotoxin单位和粘附相关基因，分别与两个物种中的抗菌药物耐药菌株。总之，弯曲杆菌菌株显示抗菌药物敏感性和毒力基因的存在之间存在统计学显著关联。
The aim of this research was to statistically analyze the association between antimicrobial susceptibility/resistance to erythromycine, gentamicin, ciprofloxacin, and tetracycline and 11 virulence genes associated with adherence, invasion, and cytotoxicity in 528 isolates of Campylobacter coli and Campylobacter jejuni obtained from retail meat and fecal samples from food-producing animals and human patients. A high percentage of Campylobacter strains were resistant to antimicrobials, specifically ciprofloxacin and tetracycline. Moreover, we observed a wide distribution of virulence genes within the analyzed strains. C. jejuni strains were more susceptible to antimicrobials, and showed greater number of virulence genes than C. coli strains. Genes related to invasion capability, such as racR, ciaB, and pldA, were associated with antimicrobial-susceptible strains in both species. The genes cdtA and dnaJ, a citotoxin unit and an adherence-related gene, respectively, were associated with antimicrobial-resistant strains in both species. In conclusion, Campylobacter strains show a statistically significant association between antimicrobial susceptibility and the presence of virulence genes.