Helicobacter pylori in the palatine tonsils of patients with IgA nephropathy compared with those of patients with recurrent pharyngotonsillitis
Helicobacter pylori in the palatine tonsils of patients with IgA nephropathy compared with those of patients with recurrent pharyngotonsillitis
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DOI：
10.1016/j.humpath.2007.04.012
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发表时间：
2007-12-01
期刊：
影响因子：
3.3
通讯作者：
Inokuchi, Akira
中科院分区：
文献类型：
--
作者：
Kusano, Kenichiro;Tokunaga, Osamu;Inokuchi, Akira
幽门螺杆菌感染是通过口服摄入获得的。然而，幽门螺杆菌在人类口腔和咽部的形态和显微定位是未知的。在本研究中，我们进行了免疫组织化学，免疫电镜，原位杂交和聚合酶链反应，以确定幽门螺杆菌在腭扁桃体的32例免疫球蛋白A肾病（IgAN）和141例复发性咽扁桃体炎（RPT）。球形幽门螺杆菌存在于扁桃体隐窝复层鳞状上皮的细菌菌落和角质层中。我们首次描述了腭扁桃体中幽门螺杆菌的形态。大多数细菌菌落是硫颗粒与以色列放线菌（A israelii），和A israelii显示出显着的共存与幽门螺杆菌（P = .011）。幽门螺杆菌在腭扁桃体的RPT组的患病率急剧增加，随着年龄的增长，但四分之一的患者被发现没有扁桃体幽门螺杆菌在成年。所有IgAN患者腭扁桃体均存在幽门螺杆菌。IgAN组幽门螺杆菌感染率高于RPT组，差异有统计学意义（P < .001）。相反，A israelii与年龄和临床诊断无关（P = .722）。总之，我们的研究结果表明，球形幽门螺杆菌存在于腭扁桃体，并可能表明，幽门螺杆菌在腭扁桃体是IgAN的抗原之一。(c)2007爱思唯尔公司All rights reserved.
Helicobacter pylori infection is acquired by oral ingestion. However, the morphology and microscopic localization of H pylori in the human oral cavity and pharynx are unknown. In the present study, we performed immunohistochemistry, immunoelectron microscopy, in situ hybridization, and polymerase chain reaction to identify H pylori in the palatine tonsils of 32 patients with immunoglobulin A nephropathy (IgAN) and 141 patients with recurrent pharyngotonsillitis (RPT). H pylori in coccoid form was present in bacterial colonies and horny layers of the stratified squamous epithelium in tonsillar crypts. We described for the first time the morphology of H pylori in palatine tonsils. Most bacterial colonies were sulfur granules with Actinomyces israelii (A israelii), and A israelii showed significant coexistence with H pylori (P = .011). The prevalence of H pylori in palatine tonsils of the RPT group increased steeply with age, but one fourth of the patients were found not to have tonsillar H pylori in adulthood. All patients with IgAN had H pylori in palatine tonsils. The prevalence of H pylori was greater in the IgAN group than in the RPT group, and the difference was statistically significant (P < .001). In contrast, A israelii was unrelated to age and clinical diagnosis (P = .722). In conclusion, our results demonstrate that H pylori in coccoid form is present in palatine tonsils and may indicate that H pylori in palatine tonsils is among the antigens causative of IgAN. (c) 2007 Elsevier Inc. All rights reserved.