Cre reporter mouse expressing a nuclear localized fusion of GFP and β-galactosidase reveals new derivatives of Pax3-expressing precursors
Cre reporter mouse expressing a nuclear localized fusion of GFP and β-galactosidase reveals new derivatives of Pax3-expressing precursors
复制标题
DOI：
10.1002/dvg.20384
复制
发表时间：
2008-04-01
期刊：
影响因子：
1.5
通讯作者：
Epstein, Jonathan A.
中科院分区：
文献类型：
--
作者：
Stoller, Jason Z.;Degenhardt, Karl R.;Epstein, Jonathan A.
一种新的小鼠cre报告菌株表达了lacZ基因与GFP融合的融合蛋白，并具有核定位信号。该结构通过Cre介导的重组从ROSA26位点表达，该重组去除ioxp侧的PGK-neo盒体，从而允许在所有组织中检测Cre活性。该报告菌株与先前的R26R和R26EGFP菌株相似，在核表达以及β -半乳糖苷酶和GFP活性的联合表达方面具有一定的优势。我们表明，使用这种新开发的报告线允许提高分辨率，检测和共定位。因此，我们报告了一个以前未被识别的静脉内皮细胞亚群，来自表达Pax3的前体。
A new Cre-reporter strain of mouse has been developed that expresses a fusion protein derived from the lacZ gene fused to GFP with a nuclear localization signal. This construct is expressed from the ROSA26 locus upon Cre-mediated recombination that removes a IoxP-flanked PGK-neo cassette, thus allowing for detection of Cre activity in all tissues. This reporter strain, which is similar to prior R26R and R26EGFP strains, has certain advantages related to the nuclear expression and the combined expression of both beta-galactosidase and GFP activities. We show that the use of this newly developed reporter line allows for enhanced resolution, detection and co-localization. Thus, we report a previously unrecognized subset of venous endothelial cells derived from Pax3 expressing precursors.