Phylogenetic analysis of hemagglutinin and neuraminidase genes of H9N2 viruses isolated from migratory ducks
Phylogenetic analysis of hemagglutinin and neuraminidase genes of H9N2 viruses isolated from migratory ducks
复制标题
DOI：
10.1023/a:1026304117797
复制
发表时间：
2003-12-01
期刊：
影响因子：
1.6
通讯作者：
Kida, H
中科院分区：
文献类型：
--
作者：
Liu, JH;Okazaki, K;Kida, H
遗传分析表明，来自野生水禽的大流行性流感毒株含有15种血凝素（HA）和9种神经氨酸酶（NA）抗原亚型。监测研究表明，H9N2亚型病毒在世界各地的家禽中流行，并可感染包括人类在内的哺乳动物物种。本文对1997-2000年从西伯利亚候鸟迁徙路线日本北海道分离的5种H9N2病毒的HA和NA基因进行了遗传分析。结果表明，这些病毒的HA和NA基因分别属于同一谱系。与A/鹌鹑/香港/ g1 /97样病毒和A/鸭/香港y280 /97样病毒相比，候鸭分离株的HA和NA与韩国鸡分离株H9N2病毒的HA和NA关系密切，提示韩国H9N2病毒可能来源于候鸭。这5株分离株的NA基因与1968年引起人间大流行的N2病毒的NA基因位于同一簇，表明前一次大流行毒株的NA基因仍在水禽水库中传播。本研究结果进一步强调了开展野鸭H9N2病毒分子流行病学监测的重要性，为今后人类流感大流行的防范工作提供更多信息。
Genetic analysis indicated that the pandemic influenza strains derived from wild aquatic birds harbor viruses of 15 hemagglutinin (HA) and 9 neuraminidase (NA) antigenic subtypes. Surveillance studies have shown that H9N2 subtype viruses are worldwide in domestic poultry and could infect mammalian species, including humans. Here, we genetically analyzed the HA and NA genes of five H9N2 viruses isolated from the migratory ducks in Hokkaido, Japan, the flyway of migration from Siberia during 1997-2000. The results showed that HA and NA genes of these viruses belong to the same lineages, respectively. Compared with those of A/quail/Hong Kong/G1/97-like and A/duck/Hong KongIY280/97-like viruses, HA and NA of the migratory duck isolates had a close relationship with those of H9N2 viruses isolated from the chicken in Korea, indicating that the Korea H9N2 viruses might be derived from the migratory ducks. The NA genes of the five isolates were located in the same cluster as those of N2 viruses, which had caused a human pandemic in 1968, indicating that the NA genes of the previous pandemic strains are still circulating in waterfowl reservoirs. The present results further emphasize the importance of carrying out molecular epidemiological surveillance of H9N2 viruses in wild ducks to obtain more information for the future human influenza pandemics preparedness.