NEUROSPORA MITOCHONDRIA CONTAIN AN ACYL-CARRIER PROTEIN
NEUROSPORA MITOCHONDRIA CONTAIN AN ACYL-CARRIER PROTEIN
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DOI：
10.1111/j.1432-1033.1988.tb14005.x
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发表时间：
1988-04-15
期刊：
影响因子：
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通讯作者：
MIKOLAJCZYK, S
中科院分区：
文献类型：
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作者：
BRODY, S;MIKOLAJCZYK, S
发现粗神经孢子虫线粒体含有一种标记为[14C]泛酸并带有酰基的蛋白质。这种蛋白质经过6000倍提纯后，其物理和化学性质与细菌和叶绿体酰基载体蛋白[ACP]非常相似。经气相色谱-质谱法测定，被酯化的主要酰基是3-羟基十四酸酯。该色氨酸氨基酸序列与细菌和叶绿体ACP肽序列具有高度的相似性。鉴于神经孢子菌具有独立的细胞质酶复合体进行脂肪酸的从头合成，讨论了该ACP在脂质代谢中的可能功能。
Mitocondria of Neurospora crassa were found to contain a protein which was labelled with [14C]pantothenic acid and which carried an acyl group. This protein, when purified 6000-fold, closely resembled the bacterial and chloroplast acyl-carrier protein(s) [ACP(s)] in its physical and chemical properties. The predominant acyl group esterified to the purified protein was 3-hydroxytetradecanoate, as determined by gas chromatographic mass spectrometry. The amino acid sequence of the tryptic peptide carrying the 4''-phosphophantetheine moiety showed a high degree of sequence similarity to the analogous bacterial and chloroplast ACP peptide sequences. The possible functions of this ACP in lipid metabolism are discussed in view of the fact that Neurospora has a separate cytoplasmic enzyme complex which carries out the de novo biosynthesis of fatty acids.