MAP kinase activation by cyclosporine A
MAP kinase activation by cyclosporine A
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DOI：
10.1006/bbrc.1997.7017
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发表时间：
1997-07-30
影响因子：
3.1
通讯作者：
Morange, M
中科院分区：
文献类型：
--
作者：
Paslaru, L;Trigon, S;Morange, M
用环孢菌素 A 对 HeLa 细胞进行短暂处理，可激活粗细胞提取物中的 MAP 激酶样活性。通过 Mono Q 柱色谱分离可以分离与 MAP 激酶 ERK1 和 ERK2 共洗脱的两种活性。这两种 MAP 激酶的激活在蛋白质印迹中通过 CsA 处理后在 SDS 电泳凝胶上出现两种新的缓慢迁移形式得到证实。在肾上皮 BSC-1 细胞和 3T3 成纤维细胞中也获得了类似的激活。 MAP 激酶激活可能是由 CsA 治疗引起的钙稳态扰动所致。 (C) 1997 年学术出版社。
Short treatment of HeLa cells with cyclosporine A led to the activation in the crude cell extracts of a MAP kinase-like activity. Fractionation by chromatography on a Mono Q column allowed the separation of two activities co-eluting with the MAP kinases ERK1 and ERK2. The activation of these two MAP kinases was demonstrated in Western Blotting by the appearance, after CsA treatment, of two new slowly migrating forms on SDS electrophoretic gels. A similar activation was also obtained in renal epithelial BSC-1 cells and 3T3 fibroblasts. MAP kinase activation might result from a perturbation of calcium homeostasis induced by CsA treatment. (C) 1997 Academic Press.