Combined metabolic activity within an atrazine-mineralizing community enriched from agrochemical factory soil
Combined metabolic activity within an atrazine-mineralizing community enriched from agrochemical factory soil
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DOI：
10.1016/j.ibiod.2007.05.004
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发表时间：
2007-12-01
影响因子：
4.8
通讯作者：
Martin-Laurent, Fabrice
中科院分区：
文献类型：
--
作者：
Kolic, Nikolina Udikovic;Hrsak, Dubravka;Martin-Laurent, Fabrice
这项工作的主要目的是描述源自农化工厂土壤的莠去津矿化群落的特征，特别是阐明可培养成员的分解代谢途径以及个体代谢和遗传潜力。一个稳定的四成员细菌群落，以菌落形态和 16S rDNA 测序为特征，能够快速将莠去津矿化为 CO2 和 NH3。两种主要生物被鉴定为节杆菌属（ATZ1和ATZ2），两种次生生物（CA1和CA2）分别属于苍白杆菌属和假单胞菌属。对群落阿特拉津降解遗传潜力的 PCR 评估揭示了 trzN、trzD、atzB 和 atzC 基因的存在。分离株 ATZ1 和 ATZ2 能够将莠去津脱氯为羟基莠去津，并含有 trzN 基因。 ATZ2进一步将羟基莠去津降解为氰尿酸并含有atzB和atzC基因，而ATZ1含有atzC但不含有atzB。分离株 CA1 和 CA2 在氰尿酸上生长并含有 trzD 基因。阿特拉津的完全降解是阿特拉津分子联合代谢攻击的结果，共享阿特拉津矿化碳和氮的群落成员之间可能存在复杂的相互作用。科学相关性：尽管有大量关于纯细菌培养物降解莠去津的报道，但细菌群落所具有的途径和莠去津降解基因组合的描述很少。在这项工作中，我们描述了从农化工厂土壤中富集的四成员莠去津矿化群落，该群落能够快速将莠去津代谢为二氧化碳。这项研究将有助于更好地了解莠去津降解群落的遗传潜力和代谢活动，这些群落通常被认为是自然环境中莠去津矿化的原因。 (C) 2007 Elsevier Ltd. 保留所有权利。
The main objective of this work was to characterize an atrazine-mineralizing community originating from agrochemical factory soil, especially to elucidate the catabolic pathway and individual metabolic and genetic potentials of culturable members. A stable four-member bacterial community, characterized by colony morphology and 16S rDNA sequencing, was rapidly able to mineralize atrazine to CO2 and NH3. Two primary organisms were identified as Arthrobacter species (ATZ1 and ATZ2) and two secondary organisms (CA1 and CA2) belonged to the genera Ochrobactrum and Pseudomonas, respectively. PCR assessment of atrazine-degrading genetic potential of the community, revealed the presence of trzN, trzD, atzB and atzC genes. Isolates ATZ1 and ATZ2 were capable of dechlorinating atrazine to hydroxyatrazine and contained the trzN gene. ATZ2 further degraded hydroxyatrazine to cyanuric acid and contained atzB and atzC genes whereas ATZ1 contained atzC but not atzB. Isolates CA1 and CA2 grew on cyanuric acid and contained the trzD gene. Complete atrazine degradation was a result of the combined metabolic attack on the atrazine molecule, and complex interactions may exist between the community members sharing carbon and nitrogen from atrazine mineralization. Scientific relevance: Despite numerous reports on atrazine degradation by pure bacterial cultures, the pathways and the atrazine-degrading gene combinations harboured by bacterial communities are only poorly described. In this work, we characterized a four-member atrazine-mineralizing community enriched from an agrochemical factory soil, which was capable of rapidly metabolizing atrazine to CO2. This study will contribute towards better understanding of the genetic potential and metabolic activities of atrazine-degrading communities, which are generally considered to be responsible for atrazine mineralization in the natural environment. (C) 2007 Elsevier Ltd. All rights reserved.