Development and Evaluation of a Western Blot Kit for Diagnosis of Schistosomiasis
Development and Evaluation of a Western Blot Kit for Diagnosis of Schistosomiasis
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用于诊断血吸虫病的蛋白质印迹试剂盒的开发和评估
DOI：
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
F. Derouin
中科院分区：
文献类型：
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作者：
A. Sulahian;Y. Garin;A. Izri;C. Verret;P. Delaunay;T. van Gool;F. Derouin
我们评价了用市售抗原条进行免疫印迹(WB)分析的性能，并与间接血凝(IHA)和间接免疫荧光(IFAT)的结果进行了比较。抗原制剂为曼氏血吸虫的粗提物。通过比较58例经寄生虫学证实的曼氏血吸虫感染者(n=12)和46例血吸虫感染者(n=46)和37例疑似血吸虫病患者(血清学结果为阳性)的血清标本，对血吸虫病的Wb谱特征进行了表征。通过检测12份健康人血清样本、67份其他已证实的蠕虫和原虫感染患者的血清样本和16份自身抗体患者的血清样本，评估了WB分析的特异性。从血吸虫病患者血清中发现6条免疫优势条带(65、70、80、95、110和120 kDa)。除100 kDa条带外，在65 kDa至120 kDa范围内出现三条或更多条带被认为是血吸虫感染的诊断，在我们的系列中具有100%的特异性。在确诊的血吸虫病患者中，WB分析的敏感性为84.5%，IFAT和IHA分别为65.5%和72.9%。对于血清学证实的病例，WB分析的敏感性为97.3%。两组患者的总敏感性和特异性分别为89.5%和100%，阳性预测值和阴性预测值分别为100%和91.3%。我们认为WB分析是血吸虫病免疫学诊断的一项有用的技术。
ABSTRACT We evaluated the performance of Western blot (WB) analysis using commercially available antigen strips and compared the results with those of indirect hemagglutination (IHA) and indirect immunofluorescence (IFAT) for the serodiagnosis of human schistosomiasis. The antigen preparation was a crude extract of Schistosoma mansoni. The WB profile characteristics of schistosomiasis were characterized by comparing the results for 58 serum samples from patients with parasitologically proven S. mansoni (n = 12) and S. haematobium (n = 46) infections and 37 individuals with probable cases of schistosomiasis but with only positive serology results. The specificity of WB analysis was assessed by testing 12 serum samples from healthy subjects, 67 serum samples from patients with other proven helminthic and protozoan infections, and 16 serum samples from patients with autoantibodies. Six immunodominant bands (65, 70, 80, 95, 110, and 120 kDa) were revealed with sera from patients with schistosomiasis. The presence of three or more bands in the range 65 to 120 kDa, with the exception of the 100-kDa band, was considered diagnostic for Schistosoma infection and had a specificity of 100% in our series. In patients with proven schistosomiasis, the sensitivity of WB analysis was 84.5%, whereas those of IFAT and IHA were 65.5 and 72.9%, respectively. For serologically proven cases, the sensitivity of WB analysis was 97.3%. The overall sensitivity and specificity for both groups of patients were 89.5 and 100%, respectively, with positive and negative predictive values of 100 and 91.3%, respectively. We conclude that WB analysis is a useful technique for the immunological diagnosis of schistosomiasis.