Binding of intrinsic factor to ileal brush border membrane in the rat.
Binding of intrinsic factor to ileal brush border membrane in the rat.
复制标题
内因子与大鼠回肠刷状缘膜的结合。
DOI：
10.1016/0006-291x(83)90995-6
复制
发表时间：
1983
影响因子：
3.1
通讯作者：
Alpers,DH
中科院分区：
文献类型：
--
作者：
Seetharam,B;Bakke,JE;Alpers,DH
大鼠回肠刷状边界膜结合游离[125I]-内在因子（IF）和IF-[57Co]钴胺素（cbl）复合物。这种结合在从大鼠胃中分离的干扰素中观察到，但在猪、狗和人胃中分离的干扰素中没有观察到。游离大鼠干扰素可阻断大鼠干扰素[57Co]cbl的结合，而猪干扰素则不能。与游离IF （Ka=0.9 × 109M−1）相比，IF-cbl复合物具有更高的亲和力（Ka=0.15 × 109M−1）。大鼠IF-cbl也能有效地与人和犬的回肠膜结合。犬回肠受体抗体可阻断大鼠、人或猪IF-[57Co]cbl与人、犬回肠膜的结合，但不影响大鼠IF-[57Co]cbl与大鼠回肠膜的结合。这些发现表明大鼠回肠受体与犬和人的回肠受体不同。
The rat ileal brush border membrane binds both free [125I]-intrinsic factor (IF) and the IF-[57Co]cobalamin (cbl) complex. This binding is observed with IF isolated from rat stomach, but not from IF isolated from hog, canine and human stomachs. The binding of rat-IF[57Co]cbl can be blocked with free rat IF but not with hog IF. The IF-cbl complex binds at a higher affinity (Ka=0.15 × 109M−1) compared to that of free IF (Ka=0.9 × 109M−1). Rat IF-cbl also binds efficiently to human and canine ileal membranes. While antibody to the canine ileal receptor blocks the binding of rat, human or hog IF-[57Co]cbl to human and canine ileal membranes, it does not affect the binding of rat IF-[57Co]cbl to rat ileal membranes. These findings demostrate that the rat ileal receptor is different from canine and human ileal receptors.