Precocious expression of the Wilms' tumor gene xWT1 inhibits embryonic kidney development in Xenopus laevis.
Precocious expression of the Wilms' tumor gene xWT1 inhibits embryonic kidney development in Xenopus laevis.
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维尔姆斯肿瘤基因 xWT1 的过早表达抑制非洲爪蟾胚胎肾脏发育。
DOI：
10.1006/dbio.1998.8989
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Vize,PD
中科院分区：
文献类型：
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作者：
Wallingford,JB;Carroll,TJ;Vize,PD
肿瘤抑制因子 WT1 已被证明在体外具有多种活性，并且是体内后肾发育所必需的。在此介绍的实验中，使用非洲爪蟾原肾作为简单的模型系统来检查非洲爪蟾 WT1 (xWT1) 在肾脏发育过程中的活性。xWT1 通过 mRNA 注射在非洲爪蟾胚胎中异位表达，并发现其抑制前肾小管发育。共聚焦显微镜证实了这一观察结果，并揭示了抑制是由于未能形成适当大小的前肾原基而不是上皮化缺陷造成的。对尾芽胚胎中原肾规范的早期分子标记 Xlim-1 表达的检查表明，在任何明显的形态发生迹象（非洲爪蟾阶段 21）之前，注射 xWT1mRNA 会抑制原肾规范。这些结果表明，xWT1 可能会抑制前肾部分中肾小管特异性基因的表达，该部分注定要形成其血管结构，即血管球。
The tumor suppressor WT1 has been demonstrated to have a wide variety of activitiesin vitroand is required for metanephric developmentin vivo.In the experiments presented here, theXenopuspronephros was used as a simple model system to examine the activity ofXenopus WT1(xWT1) during kidney development.xWT1was ectopically expressed inXenopusembryos by mRNA injection and found to inhibit pronephric tubule development. Confocal microscopy confirmed this observation and revealed that the inhibition was the result of a failure to form a pronephric anlage of appropriate size rather than a defect in epithelialization. Examination ofXlim-1expression, an early molecular marker of pronephric specification, in tailbud embryos indicated that injectedxWT1mRNA inhibited pronephric specification prior to any overt sign of morphogenesis (Xenopusstage 21). These results suggest that xWT1 may act to repress tubule-specific gene expression in the portion of the pronephros fated to form its vascular structure, the glomus.