Cryopreservation of bovine ovarian tissue:: Structural normality of follicles after thawing and culture in vitro
Cryopreservation of bovine ovarian tissue:: Structural normality of follicles after thawing and culture in vitro
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DOI：
10.1006/cryo.1999.2170
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发表时间：
1999-06-01
期刊：
影响因子：
2.7
通讯作者：
Shaw, RW
中科院分区：
文献类型：
--
作者：
Paynter, SJ;Cooper, A;Shaw, RW
从冷冻保存的卵巢组织中恢复活卵泡将有益于辅助生殖的许多领域。卵巢组织冷冻保存后需要保持结构完整性，以便取出健康的卵泡，然后在体外培养以产生可行的卵母细胞。我们评估了牛组织在暴露于二甲亚砜或冷冻保存后 0、1、4、24 或 48 小时的体外培养的效果。冷冻后，组织内初级卵泡和窦前卵泡的正常性显着低于未冷冻时暴露于冷冻保护剂的组织或对照组织。培养 3 小时后，冷冻保存的组织似乎已从冷冻造成的损伤中恢复，但培养 24 小时和 48 小时后，具有正常形态的组织百分比有所下降。对于所研究的任何培养时间，对照组织和暴露于冷冻保护剂但未冷冻的组织的正常百分比之间没有显着差异。这些数据表明，可以冷冻/解冻牛卵巢组织，同时保留形态完整的卵泡的合理产量，并且短期解冻后培养可以增强卵泡的恢复。 (C) 1999 年学术出版社。
The recovery of viable follicles from cryopreserved ovarian tissue would be of benefit in many areas of assisted reproduction. Structural integrity needs to be maintained following cryopreservation of ovarian tissue in order to retrieve healthy follicles which can then be cultured in vitro to produce viable oocytes. We have assessed the effect of in vitro culture of bovine tissue for 0, 1, 4, 24, or 48 h after exposure to, or cryopreservation in, dimethylsulphoxide. Immediately after freezing, normality of primary and preantral follicles within the tissue was significantly lower than for tissue exposed to the cryoprotectant without freezing or for control tissue. After 3 h in culture, cryopreserved tissue appeared to have recovered from damage caused by freezing, although the percentage of tissue with normal morphology declined after 24 and 48 h of culture. There was no significant difference between percentage normality in control tissue and tissue exposed to the cryoprotectant without freezing for any of the culture rimes studied. These data indicate that it is possible to freeze/thaw bovine ovarian tissue while retaining a reasonable yield of morphologically intact follicles and that a short period of post-thaw culture may enhance follicle recovery. (C) 1999 Academic Press.